The elongation stage of transcription by RNA polymerase II (RNA Pol II) is central to the
regulation of gene expression in response to developmental and environmental cues in …

Understanding the mechanisms by which chromatin structure controls eukaryotic
transcription has been an intense area of investigation for the past 25 years. Many of the key …

During gene transcription, RNA polymerase II (RNAPII) traverses nucleosomes in chromatin,
but the mechanism has remained elusive. Using cryo–electron microscopy, we obtained …

During eukaryotic transcription elongation, RNA polymerase II (RNAP2) is regulated by a
chorus of factors. Here, we identified a common binary interaction module consisting of TFIIS …

Histone modifying enzymes play critical roles in many key cellular processes and are
appealing proteins for targeting by small molecules in disease. However, while the functions …

Abstract The Mediator (Med) complex relays regulatory information from DNA-bound
transcription factors to the RNA polymerase II in eukaryotes. This macromolecular unit is …

BACKGROUND: Mediator is an evolutionarily conserved multisubunit RNA polymerase II
(Pol II) coregulatory complex. Although Mediator was initially found to play a critical role in …

Macromolecular complexes are responsible for most of the essential mechanisms in cells,
leading to a broad interest in their purification and characterization. Co-expression is now …

Abstract The Paf1 complex (Paf1C) is a conserved transcription elongation factor that
regulates transcription elongation efficiency, facilitates co-transcriptional histone …

Transcription elongation requires elaborate coordination between the transcriptional
machinery and chromatin regulatory factors to successfully produce RNA while preserving …