Single-molecule measurements provide unique information on heterogeneous populations
of molecules: They give access to the complete distribution of observables (rather than only …

Fluorescence lifetime imaging is a rather new and effective tool that can be used to study
complex biological samples, either at microscopic or macroscopic levels. The map of the …

Time-correlated single photon counting (TCSPC) is based on the detection of single photons
of a periodic light signal, measurement of the detection time of the photons, and the build-up …

Providing much-needed information on fluorescence spectroscopy and microscopy, this
ready reference covers detection techniques, data registration, and the use of spectroscopic …

We present a new method to measure absolute diffusion coefficients at nanomolar
concentrations with high precision. Based on a modified fluorescence correlation …

Publisher Summary Fluorescence lifetime imaging microscopy (FLIM) produces spatially
resolved images of fluorophore lifetime (the property describing how rapidly fluorescence …

A new method of performing fluorescence correlation spectroscopy (FCS) measurements for
mixtures of several fluorescent molecular species is introduced. It uses time-resolved …

Fluorescence correlation spectroscopy (FCS) is a powerful spectroscopic technique for
studying samples at dilute fluorophore concentrations down to single molecules. The …

This article explains the basic principles of FLCS, a genuine fusion of Time-Correlated
Single Photon Counting (TCSPC) and Fluorescence Correlation Spectroscopy (FCS), using …

Single-molecule spectroscopy has developed into a widely used method for probing the
structure, dynamics, and mechanisms of biomolecular systems, especially in combination …