Despite the pre-eminence of the mouse in modelling human disease, several aspects of
murine biology limit its routine use in large-scale genetic and therapeutic screening. Many …

Site-specific recombinase systems (Cre-loxP, Flp-FRT, and φC31-att) are transforming both
forward and reverse genetics in mice. By enabling high-fidelity DNA modifications to be …

Congenital heart disease (CHD) is the most prevalent birth defect, affecting nearly 1% of live
births; the incidence of CHD is up to tenfold higher in human fetuses,. A genetic contribution …

Genome editing using engineered nucleases is used for targeted mutagenesis. But because
genome editing does not target all loci with similar efficiencies, the mutation hit-rate at a …

To support the role of DISC1 in human psychiatric disorders, we identified and analyzed two
independently derived ENU-induced mutations in Exon 2 of mouse Disc1. Mice with …

TDP‐43 (encoded by the gene TARDBP) is an RNA binding protein central to the
pathogenesis of amyotrophic lateral sclerosis (ALS). However, how TARDBP mutations …

We genotyped 2,861 cases of primary biliary cirrhosis (PBC) from the UK PBC Consortium
and 8,514 UK population controls across 196,524 variants within 186 known autoimmune …

One of the most powerful methods available to assign function to a gene is to inactivate or
knockout the gene. Recently, we described the first target-selected knockout in zebrafish …

The zebrafish has become a favorite organism for genetic analysis of vertebrate
development, but methods for generating mutants by reverse genetic approaches have …

Over the past 15 years, more than 2,000 quantitative trait loci (QTLs) have been identified in
crosses between inbred strains of mice and rats, but less than 1% have been characterized …