The native chemical ligation reaction (NCL) involves reacting a C-terminal peptide thioester
with an N-terminal cysteinyl peptide to produce a native peptide bond between the two …

Today, Fmoc SPPS is the method of choice for peptide synthesis. Very‐high‐quality Fmoc
building blocks are available at low cost because of the economies of scale arising from …

Ribosomes can produce proteins in minutes and are largely constrained to proteinogenic
amino acids. Here, we report highly efficient chemistry matched with an automated fast-flow …

Methods to prepare proteins that include a specific modification at a desired position are
essential for understanding their cellular functions and physical properties in living systems …

pH determines selectivity: The ligation of peptide hydrazides is a new method for protein
chemical synthesis that is complementary to native chemical ligation. Peptide hydrazides …

A convergent strategy for glycoprotein synthesis entails the assembly of individual peptidyl
substrates, each bearing a single carbohydrate domain. These subunits would then be …

The central dogma of modern biology underscores the pivotal roles proteins play in diverse
biological processes, the study of which necessitates advanced methods to produce …

A thiol group introduced on the γ-carbon of lysine mediates robust native chemical ligation at
both the α-and ε-amines in two consecutive steps. Desulfurization then affords the final …

Since the introduction of recombinant protein expression in the second half of the 1970s, the
growth of the biopharmaceutical field has been rapid and protein therapeutics has come to …

Solid phase peptide synthesis (SPPS) provides the possibility to chemically synthesize
peptides and proteins. Applying the method on hydrophilic structures is usually without …