Hybrid small-molecule/protein fluorescent probes are powerful tools for visualizing protein
localization and function in living cells. These hybrid probes are constructed by diverse site …

Organic fluorophores are indispensable tools in cells, tissue and in vivo imaging, and have
enabled much progress in the wide range of biological and biomedical fields. However …

In this Primer, we focus on the most recent advancements in stimulated emission depletion
(STED) microscopy, encompassing optics, computational microscopy and probes design …

Live-cell super-resolution microscopy enables the imaging of biological structure dynamics
below the diffraction limit. Here we present enhanced super-resolution radial fluctuations …

Super‐resolution techniques like single‐molecule localisation microscopy (SMLM) and
stimulated emission depletion (STED) microscopy have been extended by the use of non …

Single-molecule localization microscopy (SMLM) has revolutionized biological imaging,
improving the spatial resolution of traditional microscopes by an order of magnitude …

How molecules interact governs how they move. Single-molecule tracking (SMT) thus
provides a unique window into the dynamic interactions of biomolecules within live cells …

Single-molecule imaging provides a powerful way to study biochemical processes in live
cells, yet it remains challenging to track single molecules while simultaneously detecting …

The advancement of fluorescence microscopy techniques has opened up new opportunities
for visualizing proteins and unraveling their functions in living biological systems. Small …

Investigating the interplay of cellular proteins with optical microscopy requires multitarget
labeling. Spectral multiplexing using high-affinity or covalent labels is limited in the number …