Stimulated emission depletion microscopy

G Lukinavičius, J Alvelid, R Gerasimaitė… - Nature Reviews …, 2024 - nature.com
In this Primer, we focus on the most recent advancements in stimulated emission depletion
(STED) microscopy, encompassing optics, computational microscopy and probes design …

Neural network-based processing and reconstruction of compromised biophotonic image data

MJ Fanous, P Casteleiro Costa, Ç Işıl… - Light: Science & …, 2024 - nature.com
In recent years, the integration of deep learning techniques with biophotonic setups has
opened new horizons in bioimaging. A compelling trend in this field involves deliberately …

Light-microscopy based dense connectomic reconstruction of mammalian brain tissue

MR Tavakoli, J Lyudchik, M Januszewski, V Vistunou… - bioRxiv, 2024 - biorxiv.org
The information-processing capability of the brain's cellular network depends on the
physical wiring pattern between neurons and their molecular and functional characteristics …

[HTML][HTML] Super-resolution microscopies, technological breakthrough to decipher mitochondrial structure and dynamic.

P Teixeira, R Galland, A Chevrollier - Seminars in Cell & Developmental …, 2024 - Elsevier
Mitochondria are complex organelles with an outer membrane enveloping a second inner
membrane that creates a vast matrix space partitioned by pockets or cristae that join the …

Harnessing artificial intelligence to reduce phototoxicity in live imaging

E Gómez-de-Mariscal, M Del Rosario… - Journal of Cell …, 2024 - journals.biologists.com
Fluorescence microscopy is essential for studying living cells, tissues and organisms.
However, the fluorescent light that switches on fluorescent molecules also harms the …

The synaptic vesicle cluster as a controller of pre‐and postsynaptic structure and function

S Reshetniak, CA Bogaciu, S Bonn… - The Journal of …, 2024 - Wiley Online Library
The synaptic vesicle cluster (SVC) is an essential component of chemical synapses, which
provides neurotransmitter‐loaded vesicles during synaptic activity, at the same time as also …

[HTML][HTML] Single-frame structured illumination microscopy for fast live-cell imaging

H Wu, Y Li, Y Sun, L Yin, W Sun, Z Ye, X Yang, H Zhu… - APL Photonics, 2024 - pubs.aip.org
Observing subcellular structural dynamics in living cells has become the goal of super-
resolution (SR) fluorescence microscopy. Among typical SRM techniques, structured …

Super-resolution imaging of the neuronal cytoskeleton

C Butler-Hallissey, C Leterrier - npj Imaging, 2024 - nature.com
The complexity of the brain organization and the unique architecture of neurons have
motivated neuroscientists to stay at the forefront of cellular microscopy and rapidly take …

Fast and Long‐Term Super‐Resolution Imaging of Endoplasmic Reticulum Nano‐structural Dynamics in Living Cells Using a Neural Network

JV Rahm, A Balakrishnan, M Wehrheim… - Small …, 2025 - Wiley Online Library
Stimulated emission depletion (STED) microscopy is a super‐resolution technique that
surpasses the diffraction limit and has contributed to the study of dynamic processes in living …

[PDF][PDF] 深度学习在超分辨显微成像中的研究进展(特邀)

鲁心怡, 黄昱, 张梓童, 吴天筱, 吴洪军… - Laser & …, 2024 - researching.cn
摘要超分辨显微成像技术打破了传统显微镜存在的衍射极限限制, 提供了前所未有的细节观察
能力, 使人们得以观察到衍射极限以下的微观世界, 有力地推动了生物医学, 细胞学 …