The roles of reactive oxygen species and antioxidants in cryopreservation

JS Len, WSD Koh, SX Tan - Bioscience reports, 2019 - portlandpress.com
Cryopreservation has facilitated advancement of biological research by allowing the storage
of cells over prolonged periods of time. While cryopreservation at extremely low …

Cold-inducible proteins CIRP and RBM3, a unique couple with activities far beyond the cold

X Zhu, C Bührer, S Wellmann - Cellular and molecular life sciences, 2016 - Springer
Cold-inducible RNA-binding protein (CIRP) and RNA-binding motif protein 3 (RBM3) are
two evolutionarily conserved RNA-binding proteins that are transcriptionally upregulated in …

Quantitative evaluation and selection of reference genes in mouse oocytes and embryos cultured in vivo and in vitro

S Mamo, AB Gal, S Bodo, A Dinnyes - BMC developmental biology, 2007 - Springer
Background Real-time PCR is an efficient tool to measure transcripts and provide valuable
quantitative information on gene expression of preimplantation stage embryos. Finding valid …

Antioxidants increase blastocyst cryosurvival and viability post-vitrification

TT Truong, DK Gardner - Human reproduction, 2020 - academic.oup.com
STUDY QUESTION What is the effect of antioxidants acetyl-L-carnitine, N-acetyl-L-cysteine
and α-lipoic acid (A3) in vitrification and warming solutions on mouse blastocyst …

Cryopreservation of mammalian oocytes and embryos: current problems and future perspectives

M Moussa, J Shu, XH Zhang, FY Zeng - Science China Life Sciences, 2014 - Springer
Cryopreservation techniques for mammalian oocytes and embryos have rapidly progressed
during the past two decades, emphasizing their importance in various assisted reproductive …

Vitrification of in vitro matured ovine oocytes affects in vitro pre‐implantation development and mRNA abundance

S Succu, D Bebbere, L Bogliolo, F Ariu… - Molecular …, 2008 - Wiley Online Library
The impact of vitrification procedures on in vitro matured (IVM) ovine oocytes mRNA content
and ability to undergo successful fertilization, cleavage and embronic development was …

[HTML][HTML] Vitrification yields higher cryo-survival rate than slow freezing in biopsied bovine in vitro produced blastocysts

V Najafzadeh, JBM Secher, M Pihl, A Ærenlund… - Theriogenology, 2021 - Elsevier
Vitrification and slow freezing are the two commonly used embryo cryopreservation
methods. In most studies, vitrification of intact embryos has proven superior in several …

Effect of vitrification on the mRNA transcriptome of bovine oocytes

N Wang, CY Li, HB Zhu, HS Hao… - … in domestic animals, 2017 - Wiley Online Library
Contents Vitrification has been shown to decrease the developmental capacity of
mammalian oocytes, and this is closely associated with the abnormal mRNA expressions of …

Recovery of mitochondrial function and endogenous antioxidant systems in vitrified bovine oocytes during extended in vitro culture

XM Zhao, WH Du, D Wang, HS Hao… - Molecular …, 2011 - Wiley Online Library
This study was designed to examine the recovery of mitochondrial function and endogenous
antioxidant systems in vitrified oocytes during extended incubations. After 16 hr of in vitro …

Live piglets derived from in vitro-produced zygotes vitrified at the pronuclear stage

T Somfai, M Ozawa, J Noguchi, H Kaneko… - Biology of …, 2009 - academic.oup.com
We report the successful cryopreservation of in vitro-produced porcine zygotes. Follicular
oocytes from prepubertal gilts were matured (IVM), fertilized (IVF), and cultured (IVC) in vitro …