In the past few years, 3D electron microscopy (3DEM) has undergone a revolution in
instrumentation and methodology. One of the central players in this wide-reaching change is …

CryoEM single-particle reconstruction has been growing rapidly over the last 3 years largely
due to the development of direct electron detectors, which have provided data with dramatic …

Macromolecular complexes are intrinsically flexible and often challenging to purify for
structure determination by single-particle cryo-electron microscopy (cryo-EM). Such …

The formal concept of a workflow to single-particle analysis of cryo-electron microscopy
(cryo-EM) images in the RELION program is described. In this approach, the structure …

CryoEM in structural biology is currently served by three public archives—EMDB for 3DEM
reconstructions, PDB for models built from 3DEM reconstructions, and EMPIAR for the raw …

In January 2020, a workshop was held at EMBL-EBI (Hinxton, UK) to discuss data
requirements for the deposition and validation of cryoEM structures, with a focus on single …

Abstract The XRCC1–DNA ligase IIIα complex (XL) is critical for DNA single-strand break
repair, a key target for PARP inhibitors in cancer cells deficient in homologous …

Electron cryo-microscopy (cryo-EM) is increasingly being used to determine 3D structures of
a broad spectrum of biological specimens from molecules to cells. Anticipating this progress …

Electron cryomicroscopy (cryoEM) is essential for the study and functional understanding of
non-crystalline macromolecules such as proteins. These molecules cannot be imaged using …

Due to the extremely low signal-to-noise ratio (SNR) and unknown poses (projection angles
and image translation) in cryo-EM experiments, reconstructing 3D structures from 2D images …