Speed scaling in multiphoton fluorescence microscopy
After over 30 years of advances, multiphoton microscopy (MPM) is now instrumental in a
wide range of in vivo biological imaging applications. However, it has, until recently …
wide range of in vivo biological imaging applications. However, it has, until recently …
Non-diffracting light wave: fundamentals and biomedical applications
The light propagation in the medium normally experiences diffraction, dispersion, and
scattering. Studying the light propagation is a century-old problem as the photons may …
scattering. Studying the light propagation is a century-old problem as the photons may …
Volumetric Ca2+ imaging in the mouse brain using hybrid multiplexed sculpted light microscopy
Calcium imaging using two-photon scanning microscopy has become an essential tool in
neuroscience. However, in its typical implementation, the tradeoffs between fields of view …
neuroscience. However, in its typical implementation, the tradeoffs between fields of view …
High-speed volumetric two-photon fluorescence imaging of neurovascular dynamics
Understanding the structure and function of vasculature in the brain requires us to monitor
distributed hemodynamics at high spatial and temporal resolution in three-dimensional (3D) …
distributed hemodynamics at high spatial and temporal resolution in three-dimensional (3D) …
Rapid mesoscale volumetric imaging of neural activity with synaptic resolution
Imaging neurons and neural circuits over large volumes at high speed and subcellular
resolution is a difficult task. Incorporating a Bessel focus module into a two-photon …
resolution is a difficult task. Incorporating a Bessel focus module into a two-photon …
A fast genetically encoded fluorescent sensor for faithful in vivo acetylcholine detection in mice, fish, worms and flies
Here we design and optimize a genetically encoded fluorescent indicator, iAChSnFR, for the
ubiquitous neurotransmitter acetylcholine, based on a bacterial periplasmic binding protein …
ubiquitous neurotransmitter acetylcholine, based on a bacterial periplasmic binding protein …
High-throughput synapse-resolving two-photon fluorescence microendoscopy for deep-brain volumetric imaging in vivo
Optical imaging has become a powerful tool for studying brains in vivo. The opacity of adult
brains makes microendoscopy, with an optical probe such as a gradient index (GRIN) lens …
brains makes microendoscopy, with an optical probe such as a gradient index (GRIN) lens …
Neurophotonic tools for microscopic measurements and manipulation: status report
Neurophotonics was launched in 2014 coinciding with the launch of the BRAIN Initiative
focused on development of technologies for advancement of neuroscience. For the last …
focused on development of technologies for advancement of neuroscience. For the last …
Three-photon fluorescence microscopy with an axially elongated Bessel focus
Volumetric imaging tools that are simple to adopt, flexible, and robust are in high demand in
the field of neuroscience, where the ability to image neurons and their networks with high …
the field of neuroscience, where the ability to image neurons and their networks with high …
In vivo volumetric imaging of calcium and glutamate activity at synapses with high spatiotemporal resolution
Studying neuronal activity at synapses requires high spatiotemporal resolution. For high
spatial resolution in vivo imaging at depth, adaptive optics (AO) is required to correct sample …
spatial resolution in vivo imaging at depth, adaptive optics (AO) is required to correct sample …