Improving protein quantity and quality—The next level of plant molecular farming

H Liu, MP Timko - International Journal of Molecular Sciences, 2022 - mdpi.com
Plants offer several unique advantages in the production of recombinant pharmaceuticals for
humans and animals. Although numerous recombinant proteins have been expressed in …

Unintended genomic outcomes in current and next generation GM techniques: A systematic review

P Chu, SZ Agapito-Tenfen - Plants, 2022 - mdpi.com
Classical genetic engineering and new genome editing techniques, especially the
CRISPR/Cas technology, increase the possibilities for modifying the genetic material in …

The synergistic effects of environmental and genetic factors on the regulation of anthocyanin accumulation in plant tissues

VG Do, Y Lee, JH Kim, YS Kwon, JT Park… - International Journal of …, 2023 - mdpi.com
Anthocyanin accumulation is responsible for the coloration of apple fruit, and their
accumulation depends on the expression of anthocyanin biosynthesis-related genes. Light …

Three parts of the plant genome: on the way to success in the production of recombinant proteins

SM Rozov, AA Zagorskaya, YM Konstantinov… - Plants, 2022 - mdpi.com
Recombinant proteins are the most important product of current industrial biotechnology.
They are indispensable in medicine (for diagnostics and treatment), food and chemical …

Applications of CRISPR/Cas9 in a rice protein expression system via an intron-targeted insertion approach

TM Nguyen, CA Lu, LF Huang - Plant Science, 2022 - Elsevier
The sugar starvation-inducible rice αAmy3 promoter and signal peptide are widely used to
produce valuable recombinant proteins in rice suspension culture cells. Conventionally, the …

Introducing MdTFL1 Promotes Heading Date and Produces Semi-Draft Phenotype in Rice

VG Do, Y Lee, S Kim, S Yang, J Park, G Do - International Journal of …, 2023 - mdpi.com
Flowering time (in rice, termed the heading date), plant height, and grain number are crucial
agronomic traits for rice productivity. The heading date is controlled via environmental …

Rice cell suspension culture as a model for producing high-value recombinant proteins and plant specialized metabolites

SS Arya, DD Kumari, JE Rookes, DM Cahill… - Plant Cell, Tissue and …, 2021 - Springer
Rice cell suspension culture (RCSC) is one of the most widely studied plant cell culture
systems next to tobacco and carrot. Simple cell culture techniques, scalability and high …

Knockdown expression of a MYB-related transcription factor gene, OsMYBS2, enhances production of recombinant proteins in rice suspension cells

DS Sinaga, SL Ho, CA Lu, SM Yu, LF Huang - Plant Methods, 2021 - Springer
Background Transgenic plant suspension cells show economic potential for the production
of valuable bioproducts. The sugar starvation-inducible rice αAmy3 promoter, together with …

High-yield BMP2 expression in rice cells via CRISPR and endogenous αAmy3 promoter

TM Nguyen, PY Wu, CH Chang, LF Huang - Applied Microbiology and …, 2024 - Springer
Plant cells serve as versatile platforms for the production of high-value recombinant proteins.
This study explored the efficacy of utilizing an endogenous αAmy3 promoter for the …

Rapid Production of Cyclic Citrullinated Peptide Monoclonal Antibody in Nicotiana benthamiana for the Early Detection and Diagnosis of Rheumatoid Arthritis

VG Do - SynBio, 2023 - mdpi.com
Rheumatoid arthritis (RA) is one of the most common autoimmune diseases, affecting 0.5%
to 1% of the population. It could ultimately result in joint destruction, functional decline, work …