Fucose is a 6-deoxy hexose in the l-configuration found in a large variety of different
organisms. In mammals, fucose is incorporated into N-glycans, O-glycans and glycolipids by …

Glycans linked to proteins and lipids play key roles in biology; thus, accurate replication of
cellular glycans is crucial for maintaining function following cell division. The fact that …

Highlights•Protein O-fucosyltransferases 1 and 2 (POFUT1 and POFUT2) are ER-localized
and modify EGF repeats and TSRs.•Both POFUT1 and POFUT2 are exquisitely selective for …

SPINDLY (SPY) in Arabidopsis thaliana is a novel nucleocytoplasmic protein O-
fucosyltransferase (POFUT), which regulates diverse developmental processes. Sequence …

Core-fucosylation is an essential biological modification by which a fucose is transferred
from GDP-β-L-fucose to the innermost N-acetylglucosamine residue of N-linked glycans. A …

Glycosylation is the most ubiquitous post-translational modification in eukaryotes. N-glycan
is attached to nascent glycoproteins and is processed and matured by various glycosidases …

Thrombospondin type 1 repeats (TSRs) occur in diverse proteins involved in adhesion and
signaling. The two extracellular TSRs of the netrin receptor UNC5A contain WxxWxxWxxC …

SPINDLY (SPY) is a novel nucleocytoplasmic protein O-fucosyltransferase that regulates
target protein activity or stability via O-fucosylation of specific Ser/Thr residues. Previous …

Highlights•GTs are highly selective in nature.•Structural snapshots provided insights into the
catalytic mechanisms of GTs.•GTs have evolved a series of mechanisms to gain functionality …

Abstract Protein O-fucosyltransferase 1 (POFUT1) fucosylates the epidermal growth factor
(EGF)-like domains found in cell-surface and secreted glycoproteins including Notch and its …