CRISPR-associated Cas12 proteins are a highly variable collection of nucleic acid-targeting
proteins. All Cas12 variants use RNA guides and a single nuclease domain to target …

The CRISPR-Cas12a system has revolutionized nucleic acid testing (NAT) with its rapid and
precise capabilities, yet it traditionally required RNA pre-amplification. Here we develop …

Nucleic acid detection powered by CRISPR technology provides a rapid, sensitive, and
deployable approach to molecular diagnostics. While exciting, there remain challenges …

Active clustered regularly interspaced short palindromic repeats (CRISPR/Cas12a) systems
possess both cis-cleavage (targeted) and trans-cleavage (collateral) activities, which are …

Many CRISPR-Cas immune systems generate guide (g) RNAs using trans-activating
CRISPR RNAs (tracrRNAs). Recent work revealed that Cas9 tracrRNAs could be …

Clostridium butyricum (Cb Ago)-based bioassays are popular due to their programmability
and directional cleavage capabilities. However, the relatively compact protein structure of …

The development of gene editing tools has been a significant area of research in the life
sciences for nearly 30 years. These tools have been widely utilized in disease detection and …

The CRISPR‐Cas12a system has emerged as a powerful tool for next‐generation nucleic
acid‐based molecular diagnostics. However, it has long been believed to be effective only …

CRISPR-Cas12a, an RNA-guided nuclease, has been repurposed for genome editing and
molecular diagnostics due to its capability of cis-cleavage on target DNA and trans-cleavage …

CRISPR/Cas12a systems have been repurposed as powerful tools for developing next-
generation molecular diagnostics due to their trans-cleavage ability. However, it was long …