For many years, structure determination of biological macromolecules by cryo-electron
microscopy (cryo-EM) was limited to large complexes or low-resolution models. With recent …

Abstract Knowledge of the three-dimensional structures of proteins and other biological
macromolecules often aids understanding of how they perform complicated tasks in the cell …

Within the last 30 years, electron energy-loss spectroscopy (EELS) has become a standard
analytical technique used in the transmission electron microscope to extract chemical and …

Human red cell AQP1 is the first functionally defined member of the aquaporin family of
membrane water channels. Here we describe an atomic model of AQP1 at 3.8 Å resolution …

Cryoelectron microscopy of biological molecules is among the hottest growth areas in
biophysics and structural biology at present, and Frank is arguably the most distinguished …

Scanning transmission electron microscopy (STEM), where a converged electron probe is
scanned over a sample's surface and an imaging, diffraction, or spectroscopic signal is …

Direct electron detectors have played a key role in the recent increase in the power of single-
particle electron cryomicroscopy (cryoEM). In this chapter, we summarize the background to …

There has been enormous progress during the last few years in the determination of three-
dimensional biological structures by single particle electron cryomicroscopy (cryoEM) …

Experienced and novice holographers receive a solid foundation in the theory and practice
of holography, the next generation of imaging technology, in this superb text. The book'show …

After a general introduction to three-dimensional electron microscopy and particularly to
electron tomography (ET), the perspectives of applying ET to native (frozen–hydrated) …