Advances in hydrogen/deuterium exchange mass spectrometry and the pursuit of challenging biological systems
EI James, TA Murphree, C Vorauer, JR Engen… - Chemical …, 2021 - ACS Publications
Solution-phase hydrogen/deuterium exchange (HDX) coupled to mass spectrometry (MS) is
a widespread tool for structural analysis across academia and the biopharmaceutical …
a widespread tool for structural analysis across academia and the biopharmaceutical …
An overview of hydrogen deuterium exchange mass spectrometry (HDX-MS) in drug discovery
Introduction: Hydrogen deuterium exchange mass spectrometry (HDX-MS) is a powerful
methodology to study protein dynamics, protein folding, protein-protein interactions, and …
methodology to study protein dynamics, protein folding, protein-protein interactions, and …
Applications of hydrogen/deuterium exchange MS from 2012 to 2014
Hydrogen/deuterium exchange (HDX) detected by mass spectrometry (MS) is extraordinarily
useful in the study of many aspects of proteins, especially the analysis of protein …
useful in the study of many aspects of proteins, especially the analysis of protein …
Molecular determinants of PI3Kγ-mediated activation downstream of G-protein–coupled receptors (GPCRs)
Phosphoinositide 3-kinase gamma (PI3Kγ) has profound roles downstream of G-protein–
coupled receptors in inflammation, cardiac function, and tumor progression. To gain insight …
coupled receptors in inflammation, cardiac function, and tumor progression. To gain insight …
Mass spectrometry methods for studying structure and dynamics of biological macromolecules
L Konermann, S Vahidi, MA Sowole - Analytical chemistry, 2014 - ACS Publications
All processes that occur within living organisms are intimately linked to biological
macromolecules such as proteins, nucleic acids, or polysaccharides. Phospholipid …
macromolecules such as proteins, nucleic acids, or polysaccharides. Phospholipid …
Using hydrogen–deuterium exchange mass spectrometry to examine protein–membrane interactions
Many fundamental cellular processes are controlled via assembly of a network of proteins at
membrane surfaces. The proper recruitment of proteins to membranes can be controlled by …
membrane surfaces. The proper recruitment of proteins to membranes can be controlled by …
Respiratory complex I in mitochondrial membrane catalyzes oversized ubiquinones
R Ikunishi, R Otani, T Masuya, K Shinzawa-Itoh… - Journal of Biological …, 2023 - ASBMB
NADH-ubiquinone (UQ) oxidoreductase (complex I) couples electron transfer from NADH to
UQ with proton translocation in its membrane part. The UQ reduction step is key to triggering …
UQ with proton translocation in its membrane part. The UQ reduction step is key to triggering …
Recombinant nepenthesin II for hydrogen/deuterium exchange mass spectrometry
The pitcher secretions of the Nepenthes genus of carnivorous plants contain a proteolytic
activity that is very useful for hydrogen/deuterium exchange mass spectrometry (HX-MS) …
activity that is very useful for hydrogen/deuterium exchange mass spectrometry (HX-MS) …
Structural mass spectrometry of membrane proteins
AS Dafun, J Marcoux - Biochimica et Biophysica Acta (BBA)-Proteins and …, 2022 - Elsevier
The analysis of proteins and protein complexes by mass spectrometry (MS) has come a long
way since the invention of electrospray ionization (ESI) in the mid 80s. Originally used to …
way since the invention of electrospray ionization (ESI) in the mid 80s. Originally used to …
Activation of ClpP protease by ADEP antibiotics: insights from hydrogen exchange mass spectrometry
MA Sowole, JA Alexopoulos, YQ Cheng… - Journal of molecular …, 2013 - Elsevier
The bacterial protease ClpP consists of 14 subunits that assemble into two stacked
heptameric rings. The central degradation chamber can be accessed via axial pores. In free …
heptameric rings. The central degradation chamber can be accessed via axial pores. In free …