Feeding the ever-growing population is a major challenge, especially in light of rapidly
changing climate conditions. Genome editing is set to revolutionize plant breeding and …

The development of CRISPR–Cas systems has sparked a genome editing revolution in
plant genetics and breeding. These sequence-specific RNA-guided nucleases can induce …

A technique for chromosomal insertion of large DNA segments is much needed in plant
breeding and synthetic biology to facilitate the introduction of desired agronomic traits and …

The prokaryote-derived CRISPR–Cas genome editing technology has altered plant
molecular biology beyond all expectations. Characterized by robustness and high target …

The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …

The elucidation of protein function and its exploitation in bioengineering have greatly
advanced the life sciences. Protein mining efforts generally rely on amino acid sequences …

Prime editors, which are CRISPR–Cas9 nickase (H840A)–reverse transcriptase fusions
programmed with prime editing guide RNAs (pegRNAs), can edit bases in mammalian cells …

Transcription-activator-like effector (TALE)-based tools for base editing of nuclear and
organellar DNA rely on double-stranded DNA deaminases, which edit substrate bases on …

Main conclusion While transgenic technology has heralded a new era in crop improvement,
several concerns have precluded their widespread acceptance. Alternative technologies …

RNA-guided programmable nucleases from CRISPR systems generate precise breaks in
DNA or RNA at specified positions. In cells, this activity can lead to changes in DNA …