Computational tools and resources for CRISPR/Cas genome editing
The past decade has witnessed a rapid evolution in identifying more versatile clustered
regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) …
regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein (Cas) …
A benchmark of algorithms for the analysis of pooled CRISPR screens
Genome-wide pooled CRISPR-Cas-mediated knockout, activation, and repression screens
are powerful tools for functional genomic investigations. Despite their increasing importance …
are powerful tools for functional genomic investigations. Despite their increasing importance …
Profiling the landscape of drug resistance mutations in neosubstrates to molecular glue degraders
Targeted protein degradation (TPD) holds immense promise for drug discovery, but
mechanisms of acquired resistance to degraders remain to be fully identified. Here, we used …
mechanisms of acquired resistance to degraders remain to be fully identified. Here, we used …
Multilayered VBC score predicts sgRNAs that efficiently generate loss-of-function alleles
G Michlits, J Jude, M Hinterndorfer, M de Almeida… - Nature …, 2020 - nature.com
CRISPR–Cas9 screens have emerged as a transformative approach to systematically probe
gene functions. The quality and success of these screens depends on the frequencies of …
gene functions. The quality and success of these screens depends on the frequencies of …
Rational targeting of a NuRD subcomplex guided by comprehensive in situ mutagenesis
Developmental silencing of fetal globins serves as both a paradigm of spatiotemporal gene
regulation and an opportunity for therapeutic intervention of β-hemoglobinopathy. The …
regulation and an opportunity for therapeutic intervention of β-hemoglobinopathy. The …
A comprehensive Bioconductor ecosystem for the design of CRISPR guide RNAs across nucleases and technologies
L Hoberecht, P Perampalam, A Lun… - Nature Communications, 2022 - nature.com
The success of CRISPR-mediated gene perturbation studies is highly dependent on the
quality of gRNAs, and several tools have been developed to enable optimal gRNA design …
quality of gRNAs, and several tools have been developed to enable optimal gRNA design …
Using traditional machine learning and deep learning methods for on-and off-target prediction in CRISPR/Cas9: a review
Z Sherkatghanad, M Abdar, J Charlier… - Briefings in …, 2023 - academic.oup.com
Abstract CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats and
CRISPR-associated protein 9) is a popular and effective two-component technology used for …
CRISPR-associated protein 9) is a popular and effective two-component technology used for …
ZNF410 represses fetal globin by singular control of CHD4
Known fetal hemoglobin (HbF) silencers have potential on-target liabilities for rational β-
hemoglobinopathy therapeutic inhibition. Here, through transcription factor (TF) CRISPR …
hemoglobinopathy therapeutic inhibition. Here, through transcription factor (TF) CRISPR …
Epigenetic Control of Translation Checkpoint and Tumor Progression via RUVBL1‐EEF1A1 Axis
Epigenetic dysregulation is reported in multiple cancers including Ewing sarcoma (EwS).
However, the epigenetic networks underlying the maintenance of oncogenic signaling and …
However, the epigenetic networks underlying the maintenance of oncogenic signaling and …
Translation efficiency driven by CNOT3 subunit of the CCR4-NOT complex promotes leukemogenesis
M Ghashghaei, Y Liu, J Ettles, G Bombaci… - Nature …, 2024 - nature.com
Protein synthesis is frequently deregulated during tumorigenesis. However, the precise
contexts of selective translational control and the regulators of such mechanisms in cancer is …
contexts of selective translational control and the regulators of such mechanisms in cancer is …