Integration of Genetic and Process Engineering for Optimized Rhamnolipid Production Using Pseudomonas putida

T Tiso, N Ihling, S Kubicki, A Biselli… - … in bioengineering and …, 2020 - frontiersin.org
Rhamnolipids are biosurfactants produced by microorganisms with the potential to replace
synthetic compounds with petrochemical origin. To promote industrial use of rhamnolipids …

Device for respiration activity measurement enables the determination of oxygen transfer rates of microbial cultures in shaken 96‐deepwell microtiter plates

R Dinger, C Lattermann, D Flitsch… - Biotechnology and …, 2022 - Wiley Online Library
Mini‐bioreactors with integrated online monitoring capabilities are well established in the
early stages of process development. Mini‐bioreactors fulfil the demand for high‐throughput …

Sterilization by flocculants in drinking water treatment

S Zhang, H Zheng, X Tang, C Zhao, C Zheng… - Chemical Engineering …, 2020 - Elsevier
In this study, the relationship between coagulation-flocculation and sterilization was studied
using two conventional flocculants, polyaluminum chloride (PAC) and poly dimethyl diallyl …

Respiration-based investigation of adsorbent-bioprocess compatibility

J Pastoors, C Baltin, J Bettmer, A Deitert… - … for Biofuels and …, 2023 - Springer
Background The efficiency of downstream processes plays a crucial role in the transition
from conventional petrochemical processes to sustainable biotechnological production …

Mass transfer and bubble hydrodynamics in stirred tank with multiple properties fluid via a CFD‐PBM method

H Chen, Z Chen, XQ Wang, XG Yi… - … Canadian Journal of …, 2024 - Wiley Online Library
The performance of a stirred bioreactor was evaluated in this study in terms of the bubble
hydrodynamics and the mass transfer efficiency, using a non‐viscous Newtonian fluid of …

Unraveling the potential and constraints associated with corn steep liquor as a nutrient source for industrial fermentations

SMW Wahjudi, T Petrzik, F Oudenne… - Biotechnology …, 2023 - Wiley Online Library
Costly complex media components such as yeast extract and peptone are still widely used
in industrial bioprocesses, despite their ill‐defined composition. Side stream products such …

Elucidation of auxotrophic deficiencies of Bacillus pumilus DSM 18097 to develop a defined minimal medium

J Müller, M Beckers, N Mußmann, J Bongaerts… - Microbial cell …, 2018 - Springer
Background Culture media containing complex compounds like yeast extract or peptone
show numerous disadvantages. The chemical composition of the complex compounds is …

Real-time monitoring and control of CHO cell apoptosis by in situ multifrequency scanning dielectric spectroscopy

F Ma, A Zhang, D Chang, OD Velev, K Wiltberger… - Process …, 2019 - Elsevier
Process control strategies based on the physiological status of cells have recently been
used to enhance mammalian cell culture productivity and robustness. In this study, we …

Development of a chemically defined medium for Paenibacillus polymyxa by parallel online monitoring of the respiration activity in microtiter plates

J Goldmanns, GA Röhling, MK Lipa, T Scholand… - BMC …, 2023 - Springer
Background One critical parameter in microbial cultivations is the composition of the
cultivation medium. Nowadays, the application of chemically defined media increases, due …

Scale‐up of a Type I secretion system in E. coli using a defined mineral medium

N Ihling, A Uhde, R Scholz, C Schwarz… - Biotechnology …, 2020 - Wiley Online Library
Secretion of heterologous proteins into the culture supernatant in laboratory strains of
Escherichia coli is possible by utilizing a Type I secretion system (T1SS). One prominent …