Synthetic biology provides a new paradigm for life science research (“build to learn”) and
opens the future journey of biotechnology (“build to use”). Here, we discuss advances of …

This review presents a brief overview of the general categories of commercially used
proteases, and critically surveys the successful strategies currently being used to improve …

Here we perform phage-assisted continuous evolution (PACE) of TEV protease, which
canonically cleaves ENLYFQS, to cleave a very different target sequence, HPLVGHM, that is …

Myriad new applications of proteases would be enabled by an ability to fine-tune substrate
specificity and activity. Herein we present a general strategy for engineering protease …

A variety of chemicals have been produced through metabolic engineering approaches, and
enhancing biosynthesis performance can be achieved by using enzymes with high catalytic …

Although bespoke, sequence-specific proteases have the potential to advance
biotechnology and medicine, generation of proteases with tailor-made cleavage specificities …

Cyclic peptides are an emerging class of molecular therapeutics that are increasingly
viewed as ideal backbones for modulation of protein–protein interactions. A split‐intein …

The biotech industry is continuously seeking for new or improved biocatalysts. The success
of these efforts is often hampered by the lack of an efficient screening assay. Thus, to be …

Site-specific proteases are important tools for in vitro and in vivo cleavage of proteins. They
are widely used for diverse applications, like protein purification, assessment of protein …

With the goal of generating new enzymes that can cleave custom sequences, this article
describes a selection strategy for evolving proteases with desirable characteristics. Positive …