Liquid–liquid extraction of biomolecules: an overview and update of the main techniques

PG Mazzola, AM Lopes, FA Hasmann… - Journal of Chemical …, 2008 - Wiley Online Library
Two‐phase systems can be exploited in separation science for the extraction/purification of
desired biomolecules. This article reviews recent experimental work on the use of aqueous …

Operative utility of salt-stable proteases of halophilic and halotolerant bacteria in the biotechnology sector

N Mokashe, B Chaudhari, U Patil - International journal of biological …, 2018 - Elsevier
Proteases are universal in existence in all organisms and are needed for metabolic activities
of the cell. Also, proteases are the paramount hydrolytic enzymes widely used in industrial …

Ionic liquid-based aqueous two-phase extraction within a microchannel system

U Novak, A Pohar, I Plazl, P Žnidaršič-Plazl - Separation and purification …, 2012 - Elsevier
Ionic liquid-based aqueous two-phase systems (IL-ATPSs) are great candidates for the
replacement of volatile organic solvents in liquid–liquid extractions. Besides, microfluidic …

Liquid–liquid extraction of biopharmaceuticals from fermented broth: trends and future prospects

NV dos Santos… - Journal of Chemical …, 2018 - Wiley Online Library
Biopharmaceuticals are one of the most important groups of biotechnological products,
representing one‐quarter of all pharmaceutical sales and providing suitable and efficient …

Optimization of PEG–salt aqueous two-phase systems by design of experiments

A Glyk, D Solle, T Scheper, S Beutel - Chemometrics and Intelligent …, 2015 - Elsevier
Since the mechanism governing the partitioning behavior of biomolecules, such as proteins
and enzymes, in polyethylene glycol (PEG)–salt aqueous two-phase systems (ATPS) is …

Partitioning and extraction protease from Aspergillus tamarii URM4634 using PEG-citrate aqueous two-phase systems

OS da Silva, MHG Gomes, RL de Oliveira… - Biocatalysis and …, 2017 - Elsevier
Abstract PEG-citrate Aqueous Two-Phase Systems (ATPS) were used to recover and
partially purify protease from Aspergillus tamarii URM4634 produced by Solid State …

Influence of the molecular weight of polymer, temperature and pH on phase diagrams of poly (ethylene glycol)+ di-potassium tartrate aqueous two-phase systems

A Barani, M Pirdashti, Z Heidari, EN Dragoi - Fluid Phase Equilibria, 2018 - Elsevier
Phase diagrams and liquid–liquid equilibrium (LLE) data for aqueous two-phase systems
(ATPSs) containing di-potassium tartrate (DPT) and poly (ethylene glycols)(PEG)(1500 and …

Optimized extraction of polygalacturonase from Aspergillus aculeatus URM4953 by aqueous two-phase systems PEG/Citrate

J de Carvalho Silva, PRL de França, TS Porto - Journal of Molecular …, 2018 - Elsevier
This study sets out to compare three techniques for extracting polygalacturonase (PG) from
Aspergillus aculeatus URM4953, namely, precipitation by organic solvent (ethanol and …

Phase equilibria of aqueous mixtures of PEG with formate salt: Effects of pH, type of cation, polymer molecular weight and temperature

M Pirdashti, A Bozorgzadeh, M Ketabi, I Khoiroh - Fluid Phase Equilibria, 2019 - Elsevier
Liquid-liquid equilibrium (LLE) data for poly (ethylene glycol)(PEG) of different molecular
weights (600, 1500, 3000, 6000)+ sodium formate (NaCHO 2), potassium formate (KCHO …

Purification of CBS 819.72 α-amylase by aqueous two-phase systems: modelling using response surface methodology

R Kammoun, H Chouayekh, H Abid, B Naili… - Biochemical engineering …, 2009 - Elsevier
The partition and purification of α-amylase from a culture supernatant of Aspergillus oryzae
CBS 819.72 was made in aqueous two-phase system (ATPS). According to bibliography …