The methylotrophic yeast Pichia pastoris has been widely reported as a suitable expression
system for heterologous protein production. The use of different phenotypes under PAOX …

Pichia pastoris is currently one of the most effective and versatile systems for the expression
of heterologous proteins. Its success is due to its powerful methanol-inducible alcohol …

The methylotrophic yeast Pichia pastoris is an established protein expression host for the
production of industrial enzymes. This yeast can be grown to very high cell densities and …

Materials science and genetic engineering have joined forces over the last three decades in
the development of so-called protein-based polymers. These are proteins, typically with …

A potential vaccine candidate, Necator americanus secretory protein (Na‐ASP1), against
hookworm infections, has been expressed in Pichia pastoris. Na‐ASP1, a 45 kDa protein …

The growing antibody market and the pressure to improve productivity as well as reduce
cost of production have fueled the development of alternative expression systems. The …

Developments in process techniques for production and recovery of heterologous proteins
with Pichia pastoris are presented. Limitations for the standard techniques are described …

Carbon source regulated promoters are well‐studied standard tools for controlling gene
expression. Acquiring control over the natural regulation of promoters is important for …

Batch‐wise sorbitol addition as a co‐substrate at the induction phase of methanol fed‐batch
fermentation by Pichia pastoris (Mut+) was proposed as a beneficial recombinant protein …

The fed-batch cultivation is in many ways a benchmark for fermentation processes, and it
has been an attractive choice for the biotechnological production of various products in the …