The interaction of cells within their microenvironmental niche is fundamental to cell
migration, positioning, growth and differentiation in order to form and maintain complex …

Biological tissues are not uniquely composed of cells. A substantial part of their volume is
extracellular space, which is primarily filled by an intricate network of macromolecules …

Biomaterials, biomedical devices and engineered cell grafts can be implanted to restitute
tissue anatomy and function. Such implants can either integrate physiologically, with no or …

The control of corneal transparency depends on the integrity of its endothelial monolayer,
which is considered nonregenerative in adult humans. In pathological situations, endothelial …

We present the first full-field optical coherence tomography (FFOCT) device capable of in
vivo imaging of the human cornea. We obtained images of the epithelial structures …

Second-harmonic generation (SHG) microscopy began to emerge as a high-resolution
optical imaging modality in the late 1990s, about 10 years after two-photon excited …

The transparency and mechanical strength of the cornea are related to the highly organized
three-dimensional distribution of collagen fibrils. It is of great interest to develop specific and …

Interfaces provide the structural basis of essential bone functions. In the hierarchical
structure of bone tissue, heterogeneities such as porosity or boundaries are found at scales …

In this review, we discuss current methods for studying ocular extracellular matrix (ECM)
assembly from the 'nano'to the 'macro'levels of hierarchical organization. Since collagen is …

Abstract Two-photon (2P) microscopy is a powerful tool for imaging and exploring label-free
biological tissues at high resolution. Although this type of microscopy has been …