Microbial ecology as a scientific field is fundamentally driven by technological advance. The
past decade's revolution in DNA sequencing cost and throughput has made it possible for …

The bacteria that can be grown in the laboratory are only a small fraction of the total diversity
that exists in nature. At all levels of bacterial phylogeny, uncultured clades that do not grow …

Single-cell RNA sequencing has recently emerged as a powerful tool for mapping cellular
heterogeneity in diseased and healthy tissues, yet high-throughput methods are needed for …

We present a droplet-based microfluidics protocol for high-throughput analysis and sorting
of single cells. Compartmentalization of single cells in droplets enables the analysis of …

Droplet microfluidics has rapidly emerged as one of the key technologies opening up new
experimental possibilities in microbiology. The ability to generate, manipulate and monitor …

In this article, we review an important cytogenetic technique-fluorescence in situ
hybridization (FISH)-which is used for obtaining spatial genomic and transcriptomic …

Most cell biology experiments are performed in bulk cell suspensions where cell secretions
become diluted and mixed in a contiguous sample. Confinement of single cells to small …

Raman-activated cell sorting (RACS) has attracted increasing interest, yet throughput
remains one major factor limiting its broader application. Here we present an integrated …

As the ability to analyze individual cells in microbial populations expands, it is becoming
apparent that isogenic microbial populations contain substantial cell-to-cell differences in …

This paper describes the microfluidic streak plate (MSP), a facile method for high-throughput
microbial cell separation and cultivation in nanoliter sessile droplets. The MSP method …