Understanding how chromatin is folded in the nucleus is fundamental to understanding its
function. Although 3D genome organization has been historically difficult to study owing to a …

Many cellular processes require large-scale rearrangements of chromatin structure.
Structural maintenance of chromosomes (SMC) protein complexes are molecular machines …

Determining how chromosomes are positioned and folded within the nucleus is critical to
understanding the role of chromatin topology in gene regulation. Several methods are …

We investigated genome folding across the eukaryotic tree of life. We find two types of three-
dimensional (3D) genome architectures at the chromosome scale. Each type appears and …

Lungs undergo mechanical strain during breathing, but how these biophysical forces affect
cell fate and tissue homeostasis are unclear. We show that biophysical forces through …

Widespread changes to DNA methylation and chromatin are well documented in cancer, but
the fate of higher-order chromosomal structure remains obscure. Here we integrated …

There is a need for methods that can image chromosomes with genome-wide coverage, as
well as greater genomic and optical resolution. We introduce OligoFISSEQ, a suite of three …

The high mobility group (HMG) transcription factor TCF-1 is essential for early T cell
development. Although in vitro biochemical assays suggest that HMG proteins can serve as …

Chromosome organization is crucial for genome function. Here, we present a method for
visualizing chromosomal DNA at super-resolution and then integrating Hi-C data to produce …

It is now widely appreciated that the spatial organization of the genome is nonrandom, and
its complex 3D folding has important consequences for many genome processes. Recent …