CRISPR-Cas technology secures sustainability through its applications: a review in green biotechnology

MA Matinvafa, S Makani, N Parsasharif, MA Zahed… - 3 Biotech, 2023 - Springer
The CRISPR-Cas system's applications in biotechnology offer a promising avenue for
addressing pressing global challenges, such as climate change, environmental pollution …

Development and application of a fast and efficient CRISPR-based genetic toolkit in Bacillus amyloliquefaciens LB1ba02

Q Xin, Y Chen, Q Chen, B Wang, L Pan - Microbial Cell Factories, 2022 - Springer
Background Bacillus amyloliquefaciens is generally recognized as food safe (GRAS)
microbial host and important enzyme-producing strain in the industry. B. amyloliquefaciens …

[HTML][HTML] Activating a dormant metabolic pathway for high-temperature l-alanine production in Bacillus licheniformis

X Han, J Liu, Y Wu, Y Yang, F Tao, P Xu - Iscience, 2023 - cell.com
Summary l-Alanine is an important amino acid widely used in food, medicine, materials, and
other fields. Here, we develop Bacillus licheniformis as an efficient l-alanine microbial cell …

Conversion of food waste into 2, 3-butanediol via thermophilic fermentation: Effects of carbohydrate content and nutrient supplementation

D Yu, J O'Hair, N Poe, Q Jin, S Pinton, Y He, H Huang - Foods, 2022 - mdpi.com
Fermentation of food waste into 2, 3-butanediol (2, 3-BDO), a high-value chemical, is
environmentally sustainable and an inexpensive method to recycle waste. Compared to …

[HTML][HTML] Establishment of the CRISPR-Cpf1 gene editing system in Bacillus licheniformis and multiplexed gene knockout

S Liu, F Xiao, Y Li, Y Zhang, Y Wang, G Shi - Synthetic and Systems …, 2025 - Elsevier
Bacillus licheniformis is a significant industrial microorganism. Traditional gene editing
techniques relying on homologous recombination often exhibit low efficiency due to their …

Efficient Autoinducible Expression of Recombinant Proteins via the DegSU Quorum Sensing System in a Robust Bacillus subtilis

H Zong, L Zhang, Y Cheng, Z Sheng… - ACS Synthetic …, 2025 - ACS Publications
DegSU quorum sensing (QS) system enables autoinducible expression of recombinant
proteins in Bacillus subtilis. However, insufficient promoter strength and a complex …

[HTML][HTML] Development and application of a CRISPR-dCpf1 assisted multiplex gene regulation system in Bacillus amyloliquefaciens LB1ba02

Q Xin, B Wang, L Pan - Microbiological Research, 2022 - Elsevier
Bacillus amyloliquefaciens LB1ba02 is generally recognized as food safe (GRAS) microbial
host and important enzyme-producing strain in the industry. However, the restriction …

Simultaneous and selective production of exopolymers and polyols by metabolically engineered Bacillus licheniformis strains

CW Song, M Kwon, H Song - Biochemical Engineering Journal, 2022 - Elsevier
Bacillus licheniformis has tremendous potential for the production of exopolymers that
include polyglutamic acid (PGA), levan and exopolysaccharide (EPS); and polyols among …

地衣芽孢杆菌的双位点荧光原位杂交检测.

吴茗花, 朱小颖, 邓锐杰, 罗爱民… - Modern Food Science …, 2022 - search.ebscohost.com
吴茗花1, 朱小颖1, 邓锐杰1, 罗爱民1*, 王西2, 王鼎3 (1. 四川大学轻工科学与工程学院,
四川成都610065)(2. 四川郎酒股份有限公司, 四川泸州646500)(3. 四川宜府春酒厂有限责任 …

Genome Editing in Bacillus Licheniformis: Current Approaches and Applications

S Raphel, PM Halami - Genome Editing in Bacteria (Part 1), 2024 - benthamdirect.com
Bacillus licheniformis has been regarded as an exceptional microbial cell factory for the
production of biochemicals and enzymes. The complete genome sequencing and …