[HTML][HTML] MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples

M Miya, RO Gotoh, T Sado - Fisheries Science, 2020 - Springer
We reviewed the current methodology and practices of the DNA metabarcoding approach
using a universal PCR primer pair MiFish, which co-amplifies a short fragment of fish DNA …

Fishing for fish environmental DNA: Ecological applications, methodological considerations, surveying designs, and ways forward

M Yao, S Zhang, Q Lu, X Chen, SY Zhang… - Molecular …, 2022 - Wiley Online Library
Vast global declines of freshwater and marine fish diversity and population abundance pose
serious threats to both ecosystem sustainability and human livelihoods. Environmental DNA …

Meta‐analysis shows both congruence and complementarity of DNA and eDNA metabarcoding to traditional methods for biological community assessment

F Keck, RC Blackman, R Bossart… - Molecular …, 2022 - Wiley Online Library
DNA metabarcoding is increasingly used for the assessment of aquatic communities, and
numerous studies have investigated the consistency of this technique with traditional …

Navigating the seven challenges of taxonomic reference databases in metabarcoding analyses

F Keck, M Couton, F Altermatt - Molecular Ecology Resources, 2023 - Wiley Online Library
Assessment of biodiversity using metabarcoding data, such as from bulk or environmental
DNA sampling, is becoming increasingly relevant in ecology, biodiversity sciences and …

Biases in bulk: DNA metabarcoding of marine communities and the methodology involved

LM van der Loos, R Nijland - Molecular Ecology, 2021 - Wiley Online Library
With the growing anthropogenic pressure on marine ecosystems, the need for efficient
monitoring of biodiversity grows stronger. DNA metabarcoding of bulk samples is …

Ecosystems monitoring powered by environmental genomics: a review of current strategies with an implementation roadmap

T Cordier, L Alonso‐Sáez… - Molecular …, 2021 - Wiley Online Library
A decade after environmental scientists integrated high‐throughput sequencing
technologies in their toolbox, the genomics‐based monitoring of anthropogenic impacts on …

[HTML][HTML] The expanding role of DNA barcodes: Indispensable tools for ecology, evolution, and conservation

MR Gostel, WJ Kress - Diversity, 2022 - mdpi.com
DNA barcoding has transformed the fields of ecology, evolution, and conservation by
providing a rapid and effective tool for species identification. The growth of DNA barcodes as …

A practical guide to DNA metabarcoding for entomological ecologists

M Liu, LJ Clarke, SC Baker, GJ Jordan… - Ecological …, 2020 - Wiley Online Library
1. DNA metabarcoding is a cost‐effective species identification approach with great potential
to assist entomological ecologists. This review presents a practical guide to help …

[HTML][HTML] DNA barcoding in herbal medicine: Retrospective and prospective

S Chen, X Yin, J Han, W Sun, H Yao, J Song… - Journal of Pharmaceutical …, 2023 - Elsevier
DNA barcoding has been widely used for herb identification in recent decades, enabling
safety and innovation in the field of herbal medicine. In this article, we summarize recent …

Improved freshwater macroinvertebrate detection from environmental DNA through minimized nontarget amplification

F Leese, M Sander, D Buchner, V Elbrecht… - Environmental …, 2021 - Wiley Online Library
DNA metabarcoding of freshwater communities typically relies on PCR amplification of a
fragment of the mitochondrial cytochrome c oxidase I (COI) gene with degenerate primers …