Recent advances in microscale electroporation

SE Choi, H Khoo, SC Hur - Chemical reviews, 2022 - ACS Publications
Electroporation (EP) is a commonly used strategy to increase cell permeability for
intracellular cargo delivery or irreversible cell membrane disruption using electric fields. In …

Microfluidic devices for the detection of pesticide residues

P Murugesan, G Raj, JA Moses - Reviews in Environmental Science and …, 2023 - Springer
Pesticides, in various forms, are used for the protection of crops. However, their residues are
detrimental to human, animal, and environmental health. Therefore, their detection …

Multiplexed high-throughput localized electroporation workflow with deep learning–based analysis for cell engineering

CA Patino, N Pathak, P Mukherjee, SH Park, G Bao… - Science …, 2022 - science.org
Manipulation of cells for applications such as biomanufacturing and cell-based therapeutics
involves introducing biomolecular cargoes into cells. However, successful delivery is a …

High throughput and highly controllable methods for in vitro intracellular delivery

J Brooks, G Minnick, P Mukherjee, A Jaberi, L Chang… - Small, 2020 - Wiley Online Library
In vitro and ex vivo intracellular delivery methods hold the key for releasing the full potential
of tissue engineering, drug development, and many other applications. In recent years, there …

Recent advances in microfluidic-based electroporation techniques for cell membranes

F Wang, S Lin, Z Yu, Y Wang, D Zhang, C Cao… - Lab on a Chip, 2022 - pubs.rsc.org
Electroporation is a fundamental technique for applications in biotechnology. To date, the
ongoing research on cell membrane electroporation has explored its mechanism, principles …

Electrotransfer for nucleic acid and protein delivery

A Muralidharan, PE Boukany - Trends in biotechnology, 2024 - cell.com
Electrotransfer of nucleic acids and proteins has become crucial in biotechnology for gene
augmentation and genome editing. This review explores the applications of electrotransfer …

Nanoscale Methods for Longitudinal Extraction of Intracellular Contents

YJ Quek, A Tay - Advanced Materials, 2024 - Wiley Online Library
Longitudinal analysis of intracellular contents including gene and protein expression is
crucial for deciphering the fundamentally dynamic nature of cells. This offers invaluable …

High-throughput microfluidics platform for intracellular delivery and sampling of biomolecules from live cells

CA Patino, P Mukherjee, EJ Berns, EH Moully, L Stan… - ACS …, 2022 - ACS Publications
Nondestructive cell membrane permeabilization systems enable the intracellular delivery of
exogenous biomolecules for cell engineering tasks as well as the temporal sampling of …

Biological mass spectrometry enables spatiotemporal 'omics: From tissues to cells to organelles

LR Pade, KE Stepler, EP Portero… - Mass spectrometry …, 2024 - Wiley Online Library
Biological processes unfold across broad spatial and temporal dimensions, and
measurement of the underlying molecular world is essential to their understanding …

[HTML][HTML] Single cell transcriptomics reveals reduced stress response in stem cells manipulated using localized electric fields

P Mukherjee, CY Peng, T McGuire, JW Hwang… - Materials Today Bio, 2023 - Elsevier
Abstract Membrane disruption using Bulk Electroporation (BEP) is a widely used non-viral
method for delivering biomolecules into cells. Recently, its microfluidic counterpart …