Overview of next‐generation sequencing technologies

BE Slatko, AF Gardner… - Current protocols in …, 2018 - Wiley Online Library
High throughput DNA sequencing methodology (next generation sequencing; NGS) has
rapidly evolved over the past 15 years and new methods are continually being …

PCR inhibition in qPCR, dPCR and MPS—mechanisms and solutions

M Sidstedt, P Rådström, J Hedman - Analytical and bioanalytical chemistry, 2020 - Springer
DNA analysis has seen an incredible development in terms of instrumentation, assays and
applications over the last years. Massively parallel sequencing (MPS) and digital PCR are …

Sequencing error profiles of Illumina sequencing instruments

N Stoler, A Nekrutenko - NAR genomics and bioinformatics, 2021 - academic.oup.com
Sequencing technology has achieved great advances in the past decade. Studies have
previously shown the quality of specific instruments in controlled conditions. Here, we …

Bioinformatics and computational tools for next-generation sequencing analysis in clinical genetics

R Pereira, J Oliveira, M Sousa - Journal of clinical medicine, 2020 - mdpi.com
Clinical genetics has an important role in the healthcare system to provide a definitive
diagnosis for many rare syndromes. It also can have an influence over genetics prevention …

Systematic evaluation of error rates and causes in short samples in next-generation sequencing

F Pfeiffer, C Gröber, M Blank, K Händler, M Beyer… - Scientific reports, 2018 - nature.com
Next-generation sequencing (NGS) is the method of choice when large numbers of
sequences have to be obtained. While the technique is widely applied, varying error rates …

Fluorescent nucleobases as tools for studying DNA and RNA

W Xu, KM Chan, ET Kool - Nature chemistry, 2017 - nature.com
Understanding the diversity of dynamic structures and functions of DNA and RNA in biology
requires tools that can selectively and intimately probe these biomolecules. Synthetic …

[HTML][HTML] Application of metagenomics in the human gut microbiome

WL Wang, SY Xu, ZG Ren, L Tao… - World journal of …, 2015 - ncbi.nlm.nih.gov
There are more than 1000 microbial species living in the complex human intestine. The gut
microbial community plays an important role in protecting the host against pathogenic …

Clip chemistry: diverse (bio)(macro) molecular and material function through breaking covalent bonds

P Shieh, MR Hill, W Zhang, SL Kristufek… - Chemical …, 2021 - ACS Publications
In the two decades since the introduction of the “click chemistry” concept, the toolbox of “click
reactions” has continually expanded, enabling chemists, materials scientists, and biologists …

Next-generation sequencing—an overview of the history, tools, and “Omic” applications

JK Kulski - Next generation sequencing-advances, applications …, 2016 - books.google.com
Next-generation sequencing (NGS) technologies using DNA, RNA, or methylation
sequencing have impacted enormously on the life sciences. NGS is the choice for large …

High-throughput sequencing and metagenomics: moving forward in the culture-independent analysis of food microbial ecology

D Ercolini - Applied and environmental microbiology, 2013 - Am Soc Microbiol
Following recent trends in environmental microbiology, food microbiology has benefited
from the advances in molecular biology and adopted novel strategies to detect, identify, and …