Hybrid small-molecule/protein fluorescent probes are powerful tools for visualizing protein
localization and function in living cells. These hybrid probes are constructed by diverse site …

Highlights•Modern microscopy methods require fluorescent labels with increased
photostability.•The photobleaching of fluorophores often involves the first triplet-excited state …

Herein, we present high-yielding, concise access to a set of xanthenium-derived, water-
soluble, low-molecular-weight photocages allowing light-controlled cargo release in the …

Optical nanoantennas are known to focus freely propagating light and reversely to mediate
the emission of a light source located at the nanoantenna hotspot. These effects were …

Single-molecule FRET (smFRET) has become a mainstream technique for studying
biomolecular structural dynamics. The rapid and wide adoption of smFRET experiments by …

Highlights•The photobleaching of heptamethine cyanines arises from a photooxidative
cleavage reaction.•Slowing cyanine photooxidation generates photochemically stable …

Light microscopy can offer certain advantages over electron microscopy in terms of
acquiring detailed insights into the biological/intra-cellular milieu. In recent years, with the …

Molecular immobilization and recognition are two key events for the development of
biosensors. The general ways for the immobilization and recognition of biomolecules …

Super-resolution fluorescence microscopy is a recently developed imaging tool for
biological researches. Several methods have been developed for detection of fluorescence …

Single-molecule fluorescence resonance energy transfer (smFRET) methods employed to
quantify time-dependent compositional and conformational changes within biomolecules …