Protein analysis by shotgun/bottom-up proteomics

Y Zhang, BR Fonslow, B Shan, MC Baek… - Chemical …, 2013 - ACS Publications
According to Genome Sequencing Project statistics (http://www. ncbi. nlm. nih.
gov/genomes/static/gpstat. html), as of February 16, 2012, complete gene sequences have …

Mass spectrometry for post-translational modifications

CE Parker, V Mocanu, M Mocanu, N Dicheva… - …, 2010 - books.google.com
Post-translational modification of proteins is important for the regulation of cellular
processes, including the cellular localization of protein, the regulation of protein function …

Facts and artifacts in proteomics of body fluids. What proteomics of saliva is telling us?

I Messana, R Inzitari, C Fanali, T Cabras… - Journal of separation …, 2008 - Wiley Online Library
This review briefly depicts several salient points of the current status of knowledge on
salivary peptidoma. It outlines the intrinsic difficulties in its characterization connected to …

Oxidized and poorly glycosylated band 3 is selectively phosphorylated by Syk kinase to form large membrane clusters in normal and G6PD-deficient red blood cells

A Pantaleo, E Ferru, G Giribaldi, F Mannu… - Biochemical …, 2009 - portlandpress.com
Oxidative events involving band 3 (Anion Exchanger 1) have been associated with RBC (red
blood cell) removal through binding of NAbs (naturally occurring antibodies); however, the …

Quantitative proteomic analysis of protein complexes: concurrent identification of interactors and their state of phosphorylation

D Pflieger, MA Jünger, M Müller, O Rinner… - Molecular & cellular …, 2008 - ASBMB
Protein complexes have largely been studied by immunoaffinity purification and (mass
spectrometric) analysis. Although this approach has been widely and successfully used it is …

An efficient method for dephosphorylation of phosphopeptides by cerium oxide

F Tan, Y Zhang, J Wang, J Wei, Y Cai… - Journal of mass …, 2008 - Wiley Online Library
In this article, an effective method for dephosphorylation of phosphopeptides by cerium
oxide is described. The dephosphorylation activity of cerium oxide was evaluated by two …

A tyrosine phosphoproteome analysis approach enabled by selective dephosphorylation with protein tyrosine phosphatase

X Liu, M Dong, Y Yao, Y Wang, J Mao, L Hu… - Analytical …, 2022 - ACS Publications
Protein tyrosine phosphorylation (pTyr) plays a prominent role in signal transduction and
regulation in all eukaryotic cells. In conventional immunoaffinity purification (IP) methods …

Assay development for the determination of phosphorylation stoichiometry using multiple reaction monitoring methods with and without phosphatase treatment …

D Domanski, LC Murphy, CH Borchers - Analytical chemistry, 2010 - ACS Publications
We have developed a phosphatase-based phosphopeptide quantitation (PPQ) method for
determining phosphorylation stoichiometry in complex biological samples. This PPQ method …

Reference-facilitated phosphoproteomics: Fast and reliable phosphopeptide validation by μLC-ESI-Q-TOF MS/MS

SY Imanishi, V Kochin, SE Ferraris, A de Thonel… - Molecular & Cellular …, 2007 - ASBMB
Recent advances in instrument control and enrichment procedures have enabled us to
quantify large numbers of phosphoproteins and record site-specific phosphorylation events …

[图书][B] Neuroproteomics

O Alzate - 2009 - taylorfrancis.com
In this, the post-genomic age, our knowledge of biological systems continues to expand and
progress. As the research becomes more focused, so too does the data. Genomic research …