We previously described a protocol for genome engineering of mammalian cultured cells
with clustered regularly interspaced short palindromic repeats and associated protein 9 …

The CRISPR/Cas9 system has been used to generate fluorescently labelled fusion proteins
by homology-directed repair in a variety of species. Despite its revolutionary success, there …

Introduction Oligodendrocytes (OLs) are the myelin-forming cells of the central nervous
system (CNS). Although OLs can be differentiated from human-induced pluripotent stem …

Functional genomics studies of the immune system require genetic manipulations that
involve both deletion of target genes and addition of elements to proteins of interest …

CRISPR technology has revolutionized the biological research world, making animals
heretofore recalcitrant to genetic manipulation, accessible to analysis of specific gene …

As a severe disease characterized by reproductive failure and respiratory distress, porcine
reproductive and respiratory syndrome (PRRS) is one of the most leading threats to the …

Tracking the localization and proximal interaction partners of endogenous proteins provides
valuable functional insight. Here, we present a protocol for CRISPR-based endogenous …

Macromolecular complexes govern the majority of biological processes and are of great
biomedical relevance as factors that perturb interaction networks underlie a number of …

Recent advances in genome editing tools, especially novel developments in the clustered
regularly interspaced short palindromic repeats associated to Cas9 nucleases …

We have previously described a protocol for genome engineering of mammalian cultured
cancer cells with CRISPR/Cas9 to generate homozygous knock-ins of fluorescent tags into …