Beta-actin (ACTB) has traditionally been regarded as an endogenous housekeeping gene
and has been widely used as a reference gene/protein in quantifying expression levels in …

Gene expression changes in neuropsychiatric and neurodegenerative disorders, and gene
responses to therapeutic drugs, provide new ways to identify central nervous system (CNS) …

Background Usually the reference genes used in gene expression analysis have been
chosen for their known or suspected housekeeping roles, however the variation observed in …

Background Real-time quantitative PCR (qPCR) is a method for rapid and reliable
quantification of mRNA transcription. Internal standards such as reference genes are used to …

Background Normalizing to housekeeping gene (HKG) can make results from quantitative
real-time PCR (qRT-PCR) more reliable. Recent studies have shown that no single HKG is …

ABSTRACT Beta-actin (ACTB), a highly conserved cytoskeleton structural protein, has been
regarded as a common housekeep gene and used as a reference gene for years. However …

Quantitative real‐time polymerase chain reaction (qPCR) has become a widely used tool in
the search for disease genes. When examining gene expression with qPCR in psychiatric …

Japanese flounder (Paralichthys olivaceus) is an important economic fish species cultured
worldwide. In this report, we compared the potentials of ten housekeeping genes as …

In determining relative gene expression by quantitative measurements of mRNA levels
using real-time quantitative PCR, internal standards such as reference genes are essential …

Background Considering the broad variation in the expression of housekeeping genes
among tissues and experimental situations, studies using quantitative RT-PCR require strict …