Emerging themes in CryoEM─ Single particle analysis image processing

JL Vilas, JM Carazo, COS Sorzano - Chemical Reviews, 2022 - ACS Publications
Cryo-electron microscopy (CryoEM) has become a vital technique in structural biology. It is
an interdisciplinary field that takes advantage of advances in biochemistry, physics, and …

Novel artificial intelligence-based approaches for ab initio structure determination and atomic model building for cryo-electron microscopy

MC DiIorio, AW Kulczyk - Micromachines, 2023 - mdpi.com
Single particle cryo-electron microscopy (cryo-EM) has emerged as the prevailing method
for near-atomic structure determination, shedding light on the important molecular …

[PDF][PDF] On bias, variance, overfitting, gold standard and consensus in single-particle analysis by cryo-electron microscopy

COS Sorzano, A Jiménez-Moreno… - … Section D: Structural …, 2022 - journals.iucr.org
Cryo-electron microscopy (cryoEM) has become a well established technique to elucidate
the 3D structures of biological macromolecules. Projection images from thousands of …

Structural insights into the role of diphthamide on elongation factor 2 in mRNA reading-frame maintenance

S Pellegrino, N Demeshkina… - Journal of molecular …, 2018 - Elsevier
One of the most critical steps of protein biosynthesis is the coupled movement of mRNA,
which encodes genetic information, with tRNAs on the ribosome. In eukaryotes, this process …

Advances in xmipp for cryo–electron microscopy: From xmipp to scipion

D Strelak, A Jiménez-Moreno, JL Vilas… - Molecules, 2021 - mdpi.com
Xmipp is an open-source software package consisting of multiple programs for processing
data originating from electron microscopy and electron tomography, designed and managed …

[HTML][HTML] Improvements on marker-free images alignment for electron tomography

COS Sorzano, F de Isidro-Gómez… - Journal of Structural …, 2020 - Elsevier
Electron tomography is a technique to obtain three-dimensional structural information of
samples. However, the technique is limited by shifts occurring during acquisition that need to …

The cryo-EM structure of a novel 40S kinetoplastid-specific ribosomal protein

JB Querido, E Mancera-Martínez, Q Vicens, A Bochler… - Structure, 2017 - cell.com
Kinetoplastids are potentially lethal protozoan pathogens affecting more than 20 million
people worldwide. There is a critical need for more specific targets for the development of …

[HTML][HTML] Unveiling Liquid-Crystalline Lipids in the Urothelial Membrane through Cryo-EM

H Yanagisawa, Y Kita, T Oda, M Kikkawa - bioRxiv, 2023 - ncbi.nlm.nih.gov
The urothelium, a distinct epithelial tissue lining the urinary tract, serves as an essential
component in preserving urinary tract integrity and thwarting infections. The asymmetric unit …

[HTML][HTML] Unveiling Liquid-Crystalline Lipids in the Urothelial Membrane through Cryo-EM

T Oda, H Yanagisawa, M Kikkawa, Y Kita - Research Square, 2023 - ncbi.nlm.nih.gov
The urothelium, a distinct epithelial tissue lining the urinary tract, serves as an essential
component in preserving urinary tract integrity and thwarting infections. The asymmetric unit …

Structural basis for cooperativity of human monoclonal antibodies to meningococcal factor H-binding protein

I Peschiera, M Giuliani, F Giusti, R Melero… - Communications …, 2019 - nature.com
Monoclonal antibody (mAb) cooperativity is a phenomenon triggered when mAbs couples
promote increased bactericidal killing compared to individual partners. Cooperativity has …