Reprogramming the genetic code
D de la Torre, JW Chin - Nature Reviews Genetics, 2021 - nature.com
The encoded biosynthesis of proteins provides the ultimate paradigm for high-fidelity
synthesis of long polymers of defined sequence and composition, but it is limited to …
synthesis of long polymers of defined sequence and composition, but it is limited to …
Cracking the code: reprogramming the genetic script in prokaryotes and eukaryotes to harness the power of noncanonical amino acids
C Jann, S Giofré, R Bhattacharjee, EA Lemke - Chemical Reviews, 2024 - ACS Publications
Over 500 natural and synthetic amino acids have been genetically encoded in the last two
decades. Incorporating these noncanonical amino acids into proteins enables many …
decades. Incorporating these noncanonical amino acids into proteins enables many …
Genetic code expansion: a brief history and perspective
MA Shandell, Z Tan, VW Cornish - Biochemistry, 2021 - ACS Publications
Since the establishment of site-specific mutagenesis of single amino acids to interrogate
protein function in the 1970s, biochemists have sought to tailor protein structure in the native …
protein function in the 1970s, biochemists have sought to tailor protein structure in the native …
Root of the tree: the significance, evolution, and origins of the ribosome
The ribosome is an ancient molecular fossil that provides a telescope to the origins of life.
Made from RNA and protein, the ribosome translates mRNA to coded protein in all living …
Made from RNA and protein, the ribosome translates mRNA to coded protein in all living …
Ribosome-mediated polymerization of long chain carbon and cyclic amino acids into peptides in vitro
Ribosome-mediated polymerization of backbone-extended monomers into polypeptides is
challenging due to their poor compatibility with the translation apparatus, which evolved to …
challenging due to their poor compatibility with the translation apparatus, which evolved to …
Expanding the limits of the second genetic code with ribozymes
The site-specific incorporation of noncanonical monomers into polypeptides through genetic
code reprogramming permits synthesis of bio-based products that extend beyond natural …
code reprogramming permits synthesis of bio-based products that extend beyond natural …
A call for caution in analysing mammalian co-transfection experiments and implications of resource competition in data misinterpretation
Transient transfections are routinely used in basic and synthetic biology studies to unravel
pathway regulation and to probe and characterise circuit designs. As each experiment has a …
pathway regulation and to probe and characterise circuit designs. As each experiment has a …
In vitro ribosome synthesis and evolution through ribosome display
MJ Hammerling, BR Fritz, DJ Yoesep, DS Kim… - Nature …, 2020 - nature.com
Directed evolution of the ribosome for expanded substrate incorporation and novel functions
is challenging because the requirement of cell viability limits the mutations that can be …
is challenging because the requirement of cell viability limits the mutations that can be …
Understanding resource competition to achieve predictable synthetic gene expression in eukaryotes
Synthetic gene expression in engineered cells typically depends on the host cell's limited
pool of intracellular resources, which can lead to resource competition between native and …
pool of intracellular resources, which can lead to resource competition between native and …
Synthetic biological circuits within an orthogonal central dogma
A Costello, AH Badran - Trends in biotechnology, 2021 - cell.com
Synthetic biology strives to reliably control cellular behavior, typically in the form of user-
designed interactions of biological components to produce a predetermined output …
designed interactions of biological components to produce a predetermined output …