DNA looping can enhance lysogenic CI transcription in phage lambda

LM Anderson, H Yang - Proceedings of the National …, 2008 - National Acad Sciences
The lysogenic state of bacteriophage lambda is maintained by CI repressor, which
negatively regulates two promoters to block lytic gene expression. Expression of CI is itself …

Physicochemical properties and distinct DNA binding capacity of the repressor of temperate Staphylococcus aureus phage φ11

T Ganguly, M Das, A Bandhu, PK Chanda… - The FEBS …, 2009 - Wiley Online Library
The repressor protein and cognate operator DNA of any temperate Staphylococcus aureus
phage have not been investigated in depth, despite having the potential to enrich the …

The C-terminal domain of the Escherichia coli RNA polymerase α subunit plays a role in the CI-dependent activation of the bacteriophage λ pM promoter

B Kedzierska, A Szambowska… - Nucleic acids …, 2007 - academic.oup.com
The bacteriophage λ p M promoter is required for maintenance of the λ prophage in
Escherichia coli, as it facilitates transcription of the cI gene, encoding the λ repressor (CI). CI …

Improved Model of a LexA Repressor Dimer Bound to recA Operator

R Chattopadhyaya, A Pal - Journal of Biomolecular Structure and …, 2004 - Taylor & Francis
A complete three dimensional model for the LexA repressor dimer bound to the recA
operator site consistent with relevant biochemical and biophysical data for the repressor was …

Cloning and sequencing analysis of the repressor gene of temperate mycobacteriophage L1

S Sau, P Chattoraj, T Ganguly, CY Lee, NC Mandal - BMB Reports, 2004 - koreascience.kr
The wild-type and temperature-sensitive (ts) repressor genes were cloned from the
temperate mycobacteriophage L1 and its mutant L1cIts391, respectively. A sequencing …

pH-dependent autocleavage of lambda repressor occurs in the operator-bound form: characterization of lambda repressor autocleavage

K Ghosh, A Pal, R Chattopadhyaya - Biochemical Journal, 2004 - portlandpress.com
The first-order rate constants for the RecA-independent, spontaneous, pH-dependent
autocleavage of the λ cI repressor was measured in the present study at pH 10.6 at 27, 37 …

Effects of physical, ionic, and structural factors on the binding of repressor of mycobacteriophage L1 to its cognate operator DNA

T Ganguly, PK Chanda, A Bandhu… - Protein and Peptide …, 2006 - ingentaconnect.com
To determine the factors influencing the binding of L1 repressor to its cognate operator DNA,
several gel shift as well as bioinformatic analyses have been carried out. The data show that …

Digestion of the λ cI Repressor with Various Serine Proteases and Correlation with Its Three Dimensional Structure

A Pal, R Chattopadhyaya - Journal of Biomolecular Structure and …, 2008 - Taylor & Francis
Partial proteolysis of the λ cI repressor has been carried out systematically with trypsin,
chymotrypsin, elastase, endoproteinase Glu-C, kallikrein, and thrombin. The cleavage sites …

RecA-Mediated Cleavage of λ cI Repressor Accepts Repressor Dimers: Probable Role of Prolyl Cis-Trans Isomerization and Catalytic Involvement of H163, K177 …

A Pal, R Chattopadhyaya - Journal of Biomolecular Structure and …, 2009 - Taylor & Francis
The λ cI repressor is found to be cleaved in the presence of activated RecA in its DNA-bound
dimeric form at a rate similar to that in the absence of operator DNA in contrast to previous …

Biochemical characterization of L1 repressor mutants with altered operator DNA binding activity

A Bandhu, T Ganguly, B Jana, A Chakravarty… - …, 2012 - Taylor & Francis
A mycobacteriophage-specific repressor with the enhanced operator DNA binding activity at
32° C and no activity at 42° C has not been generated yet though it has potential in …