Enzyme co-immobilization: Always the biocatalyst designers' choice… or not?

S Arana-Peña, D Carballares… - Biotechnology …, 2021 - Elsevier
The increasing relevance of cascade reactions in biocatalysis has sparked a great interest
for enzyme co-immobilization. Enzyme co-immobilization allows access to some kinetic …

Polyethylenimine: a very useful ionic polymer in the design of immobilized enzyme biocatalysts

JJ Virgen-Ortiz, JCS Dos Santos… - Journal of materials …, 2017 - pubs.rsc.org
This review discusses the possible roles of polyethylenimine (PEI) in the design of improved
immobilized biocatalysts from diverse perspectives. This includes their use to activate …

Detailed Analysis of Galactooligosaccharides Synthesis with β-Galactosidase from Aspergillus oryzae

P Urrutia, B Rodriguez-Colinas… - Journal of Agricultural …, 2013 - ACS Publications
The synthesis of galactooligosaccharides (GOS) catalyzed by β-galactosidase from
Aspergillus oryzae (Enzeco) was studied. Using 400 g/L of lactose and 15 U/mL, maximum …

Recent advances in the production, analysis, and application of galacto-oligosaccharides

MW Iqbal, T Riaz, S Mahmood, H Liaqat… - Food Reviews …, 2023 - Taylor & Francis
Galacto-oligosaccharides (GOS) are the prebiotic constituents with various functional
qualities that promote the optimal proliferation of gut microbiota and are consequently …

Whey upgrading by enzyme biocatalysis

A Illanes - Electronic journal of biotechnology, 2011 - SciELO Chile
Whey is a co-product of processes for the production of cheese and casein that retains most
of the lactose content in milk. World production of whey is estimated around 200 million tons …

Biocatalytic strategies in the production of galacto-oligosaccharides and its global status

PS Panesar, R Kaur, RS Singh, JF Kennedy - International journal of …, 2018 - Elsevier
Galactooligosaccharides (GOSs) are the non-digestible carbohydrates that are composed of
3–10 or longer molecules of galactose and the terminal glucose molecule. These are …

Rational design of the beta-galactosidase from Aspergillus oryzae to improve galactooligosaccharide production

X Gao, J Wu, D Wu - Food chemistry, 2019 - Elsevier
The β-galactosidase from Aspergillus oryzae produces galactooligosaccharides with
beneficial prebiotic effects through the transglycosylation of lactose. However, its low …

Potential applications of carbohydrases immobilization in the food industry

FJ Contesini, J de Alencar Figueira… - International Journal of …, 2013 - mdpi.com
Carbohydrases find a wide application in industrial processes and products, mainly in the
food industry. With these enzymes, it is possible to obtain different types of sugar syrups (viz …

Immobilization of β-galactosidase from Kluyveromyces lactis onto polymeric membrane surfaces: effect of surface characteristics

HA Güleç - Colloids and Surfaces B: Biointerfaces, 2013 - Elsevier
The aim of this study was to investigate the effects of surface characteristics of plain and
plasma modified cellulose acetate (CA) membranes on the immobilization yield of β …

Mechanically stable egg white protein based immobilization carrier for β-D-galactosidase: Thermodynamics and application in whey lactose hydrolysis

MI Wahba - Reactive and Functional Polymers, 2020 - Elsevier
Abstract β-D-Galactosidase (β-gal), a crucial enzyme in food industries, was immobilized
onto egg white protein (EWP) based covalent immobilization carriers of escalated …