Directly observing enzyme catalysis in real time at the molecular level has been a long-
standing goal of structural enzymology. Time-resolved serial crystallography methods at …

The blue-light photoreceptor photoactive yellow protein (PYP) undergoes a self-contained
light cycle. The atomic structure of the bleached signaling intermediate in the light cycle of …

Publisher Summary Cooperativity in protein or enzyme systems commonly refers to ligand
binding, with the type of positive cooperativity, that gives rise to the sigmoid curves of oxygen …

Time‐resolved macromolecular crystallography unifies structure determination with
chemical kinetics, since the structures of transient states and chemical and kinetic …

A renewed interest in the Laue diffraction technique has been brought about by the
development of new, more intense and brilliant synchrotron sources along with their …

Site-directed mutagenesis and Laue diffraction data to 2.5 Å resolution were used to solve
the structures of two sequential intermediates formed during the catalytic actions of isocitrate …

The structure of a rate-limited product complex formed during a single initial round of
turnover by isocitrate dehydrogenase has been determined. Photolytic liberation of either …

Time-resolved X-ray diffraction provides direct information on three-dimensional structures
of reacting molecules and thus can be used to elucidate structural dynamics of chemical and …

A wide assortment of caged compounds, which are species whose biological activity can be
unleashed with light, have been synthesized and used to investigate a variety of biological …

Photoreleasable molecules are important in studies of various biological phenomena,
especially cell signaling. Here we report a generally applicable approach …