Gram-negative bacteria are attractive hosts for recombinant protein production because they
are fast growing, easy to manipulate, and genetically stable in large cultures. However, the …

Many pathogens use Type III and Type IV protein secretion systems to secrete virulence
factors from the bacterial cytosol into host cells. These systems operate through a one-step …

Infection with obligatory intracellular bacteria is difficult to treat, as intracellular targets and
delivery methods of therapeutics are not well known. Ehrlichia translocated factor-1 (Etf-1), a …

Vibrio cholerae can cause pandemic cholera in humans. The bacterium resides in aquatic
environments worldwide. Identification of risk factors of V. cholerae in aquatic products is …

Background Protein secretion in bacteria is an attractive strategy for heterologous protein
production because it retains the high titers and tractability of bacterial hosts while …

Abstract Background Type I interferons (IFN-I)—a group of cytokines with
immunomodulatory, antiproliferative, and antiviral properties—are widely used as …

Background Many valuable biopharmaceutical and biotechnological proteins have been
produced in Escherichia coli, however these proteins are almost exclusively localised in the …

Protein production strategies in bacteria are often limited due to the need for cell lysis and
complicated purification schemes. To avoid these challenges, researchers have developed …

The Type III Secretion System (T3SS) is a multimeric protein complex composed of over 20
different proteins, utilized by Gram-negative bacteria to infect eukaryotic host cells. The …

Proteins comprise a multibillion-dollar industry in enzymes and therapeutics, but bacterial
protein production can be costly and inefficient. Proteins of interest (POIs) must be extracted …