The free energy and conformational landscape of biomolecular systems as well as
biochemical reactions depend not only on temperature and pressure, but also on the …

The explosive growth of single-molecule techniques is transforming our understanding of
biology, helping to develop new physics inspired by emergent biological processes, and …

The effects of crowding in biological environments on biomolecular structure, dynamics, and
function remain not well understood. Computer simulations of atomistic models of …

The first protein‐binding allosteric RNA‐cleaving DNAzyme (RCD) obtained by direct in vitro
selection against eosinophil peroxidase (EPX), a validated marker for airway eosinophilia, is …

Microscale thermophoresis (MST) is a versatile technique to measure binding affinities of
binder–ligand systems, based on the directional movement of molecules in a temperature …

Direct tracing of small extracellular vesicle (sEV) cargoes holds unprecedented importance
for elucidating the mechanisms involved in intercellular communication. However, high …

We present a unified conceptual framework and the associated software package for single-
molecule Förster resonance energy transfer (smFRET) analysis from single-photon arrivals …

Mechanical forces are crucial for biological processes such as T cell antigen recognition. A
suite of molecular tension probes to measure pulling forces have been reported over the …

G-quadruplex (G4) structures assemble from guanine-rich DNA sequences and are believed
to regulate several key cellular processes. G4 formation and conformational …

The cellular environment is highly crowded, with up to 40% of the volume fraction of the cell
occupied by various macromolecules. Most laboratory experiments take place in dilute …