The applications of Western/immunoblotting (WB) techniques have reached multiple layers
of the scientific community and are now considered routine procedures in the field of …

Attaining true quantitative data from WB requires that all the players involved in the
procedure are quality controlled including the user. Appropriate protein extraction method …

Western blotting is one of the most commonly used laboratory techniques for identifying
proteins and semi-quantifying protein amounts; however, several recent findings suggest …

Western blotting is an analytical method widely used for detecting and (semi‐) quantifying
specific proteins in given samples. Western blots are continuously applied and developed …

It is currently a routine practice to require a measurement of a housekeeping reference,
including actin, glyceraldehyde-3-phosphate dehydrogenase, β-tubulin, among others, in …

Children exposed to abuse or neglect show abnormal hippocampal development and
similar findings have been reported in rodent models. Using brief daily separation (BDS), a …

Calorie restriction (CR) increases lifespan and improves brain health in mice. Ad libitum low-
protein, high-carbohydrate (LPHC) diets also extend lifespan, but it is not known whether …

Childhood maltreatment is associated with a wide range of psychopathologies including
anxiety that emerge in childhood and in many cases persist in adulthood. Increased …

Western blotting (WB), also known as immunoblotting, is a well-known molecular biology
method that biologists often use to investigate many features of the protein, ranging from …

Genetic alterations impacting ubiquitously expressed proteins involved in RNA metabolism
often result in neurodegenerative conditions, with increasing evidence suggesting that …