Biorefinery of biomass-based biofuels and biochemicals by microorganisms is a competitive
alternative of traditional petroleum refineries. Zymomonas mobilis is a natural ethanologen …

Non-coding RNAs (ncRNAs) are versatile and powerful controllers of gene expression that
have been increasingly linked to cellular metabolism and phenotype. In bacteria, identified …

There has been an absence of an efficient method of gene knockdown in the important
human pathogen Staphylococcus aureus like RNA interference in eukaryotes. The …

In bacteria and archaea, small RNAs (sRNAs) regulate complex networks through antisense
interactions with target mRNAs in trans, and riboswitches regulate gene expression in cis …

Conditionally essential (CE) genes are required by pathogenic bacteria to establish and
maintain infections. CE genes encode virulence factors, such as secretion systems and …

Multi-target regulators represent a largely untapped area for metabolic engineering and anti-
bacterial development. These regulators are complex to characterize because they often act …

sRNAs represent a powerful class of regulators that influences multiple mRNA targets in
response to environmental changes. However, very few direct sRNA–sRNA interactions …

Trans-regulating elements such as noncoding RNAs are crucial in modifying cells, and has
shown broad application in synthetic biology, metabolic engineering and RNA therapies …

Multitargeting small regulatory RNAs (sRNAs) represent a potentially useful tool for
metabolic engineering applications. Natural multitargeting sRNAs govern bacterial gene …

Current approaches to design efficient antisense RNAs (asRNAs) rely primarily on a
thermodynamic understanding of RNA–RNA interactions. However, these approaches …