CRISPR-Cas gene editing has revolutionized experimental molecular biology over the past
decade and holds great promise for the treatment of human genetic diseases. Here we …

Our ability to edit genomes lags behind our capacity to sequence them, but the growing
understanding of CRISPR biology and its application to genome, epigenome and …

Transcriptome engineering applications in living cells with RNA-targeting CRISPR effectors
depend on accurate prediction of on-target activity and off-target avoidance. Here we design …

CRISPR–Cas13 systems have recently been used for targeted RNA degradation in various
organisms. However, collateral degradation of bystander RNAs has limited their in vivo …

Abstract Background The CRISPR-Cas13 system is an RNA-guided RNA-targeting system
and has been widely used in transcriptome engineering with potentially important clinical …

Highlights•CRISPR–Cas13 is potentially capable of collateral RNA cleavage in eukaryotic
cells.•Target RNA levels, cell size, and cell-type-specific properties may influence the …

Cas13 can be used for the knockdown, editing, imaging or detection of RNA and for RNA-
based gene therapy. Here by using RNA immunoprecipitation sequencing, transcriptome …

CRISPR‐Cas13 holds substantial promise for tissue repair through its RNA editing
capabilities and swift catabolism. However, conventional delivery methods fall short in …

The discovery of Clustered Regularly Interspaced Palindromic Repeats (CRISPR) and its
associated Cas endonucleases in bacterial and archaeal species allowed scientists to …

Background RNA viruses account for many human diseases and pandemic events but are
often not targetable by traditional therapeutics modalities. Here, we demonstrate that adeno …