The genetically encoded fluorescent sensors convert chemical and physical signals into
light. They are powerful tools for the visualisation of physiological processes in living cells …

Ever since the inception of light microscopy, the laws of physics have seemingly thwarted
every attempt to visualize the processes of life at its most fundamental, sub-cellular, level …

The rhodamine system is a flexible framework for building small‐molecule fluorescent
probes. Changing N‐substitution patterns and replacing the xanthene oxygen with a …

Photoswitchable fluorescent probes are key elements of newly developed super-resolution
fluorescence microscopy techniques that enable far-field interrogation of biological systems …

Genetically-encoded biosensors based on a single fluorescent protein are widely used to
visualize analyte levels or enzymatic activities in cells, though usually to monitor relative …

Highlights•Spectral and biochemical diversity of GFP-like proteins continues to
increase.•GFP-unrelated fluorescent proteins open new areas of applications.•Red …

Optical fluorescence microscopy plays a pivotal role in the exploration of biological structure
and dynamics, especially on live specimens. Progress in the field relies, on the one hand, on …

Reversibly switchable fluorescent proteins (RSFPs) can be effectively used for super-
resolution optical fluctuation imaging (SOFI) based on the switching and fluctuation of single …

“Smart fluorophores”, such as reversibly switchable fluorescent proteins, are crucial for
advanced fluorescence imaging. However, only a limited number of such labels is available …

Super-resolution optical microscopy is a rapidly evolving scientific field dedicated to imaging
sub-wavelength-sized objects, leaving its mark in multiple branches of biology and …