Chromatin accessibility, or the physical access to chromatinized DNA, is a widely studied
characteristic of the eukaryotic genome. As active regulatory DNA elements are generally …

When fully extended, one copy of the three billion base pair human genome reaches a
length of over two meters. Yet, it must be packaged into the nucleus of a cell with an average …

The X-ray crystal structure of the nucleosome core particle of chromatin shows in atomic
detail how the histone protein octamer is assembled and how 146 base pairs of DNA are …

The exact positions of nucleosomes along genomic DNA can influence many aspects of
chromosome function. However, existing methods for mapping nucleosomes do not provide …

Publisher Summary This chapter describes the preparation method of nucleosome core
particles (NCPs) from recombinant histones. The ability to make defined NCPs, or arrays of …

In vitro studies on nucleosome core particles (NCPs) and nucleosomes have generally been
limited to the use of histone proteins isolated from chromatin. Numerous reliable and well …

We have developed a self-assembly system for nucleosome arrays in which recombinant,
post-translationally unmodified histone proteins are combined with DNA of defined …

The four core histone proteins, H2A, H2B, H3, and H4 of Xenopus laevis have been
individually expressed in milligram quantities in Escherichia coli. The full-length proteins …

Chromatin disruption and modification are associated with transcriptional regulation by
diverse coactivators and corepressors. Here we discuss the possible structural basis and …

1. INTRODUCTION In eukaryotic cells, inheritable information is stored in a nucleoprotein
complex referred to as chromatin. 1 This genome architecture serves two key purposes. On …