Hydrogen/deuterium exchange mass spectrometry in the world of small molecules

A Damont, A Legrand, C Cao… - Mass Spectrometry …, 2023 - Wiley Online Library
The combined use of hydrogen/deuterium exchange (HDX) and mass spectrometry (MS),
referred to as HDX‐MS, is a powerful tool for exploring molecular edifices and has been …

In‐droplet hydrogen–deuterium exchange to examine protein/peptide solution conformer heterogeneity

D Sharif, M Rahman, S Mahmud… - Rapid …, 2023 - Wiley Online Library
Rationale Many different structure analysis techniques are not capable of probing the
heterogeneity of solution conformations. Here, we examine the ability of in‐droplet hydrogen …

Characterizing Multidevice Capillary Vibrating Sharp-Edge Spray Ionization for In-Droplet Hydrogen/Deuterium Exchange to Enhance Compound Identification

A DeBastiani, SN Majuta, D Sharif, K Attanayake… - ACS …, 2021 - ACS Publications
Multidevice capillary vibrating sharp-edge spray ionization (cVSSI) source parameters have
been examined to determine their effects on conducting in-droplet hydrogen/deuterium …

Effect of pH on in-electrospray hydrogen/deuterium exchange of carbohydrates and peptides

JB Hatvany, OT Liyanage… - Journal of the American …, 2024 - ACS Publications
Carbohydrates are critical for cellular functions as well as an important class of metabolites.
Characterizing carbohydrate structures is a difficult analytical challenge due to the presence …

Hydrogen/deuterium exchange for the analysis of carbohydrates

JB Hatvany, ES Gallagher - Carbohydrate research, 2023 - Elsevier
Carbohydrates and glycans are integral to many biological processes, including cell-cell
recognition and energy storage. However, carbohydrates are often difficult to analyze due to …

Distinguishing between isobaric ions using microdroplet hydrogen–deuterium exchange mass spectrometry

X Song, J Li, M Mofidfar, RN Zare - Metabolites, 2021 - mdpi.com
Isobaric ions having the same mass-to-charge ratio cannot be separately identified by mass
spectrometry (MS) alone, but this limitation can be overcome by using hydrogen–deuterium …

Combining Field-Enabled Capillary Vibrating Sharp-Edge Spray Ionization with Microflow Liquid Chromatography and Mass Spectrometry to Enhance 'Omics …

SN Majuta, A DeBastiani, P Li… - Journal of the American …, 2021 - ACS Publications
Field-enabled capillary vibrating sharp-edge spray ionization (cVSSI) has been combined
with high-flow liquid chromatography (LC) and mass spectrometry (MS) to establish current …

Structure Characterization of a Disordered Peptide Using In-Droplet Hydrogen/Deuterium Exchange Mass Spectrometry and Molecular Dynamics

MA Rahman, MN Sultana, D Sharif… - ACS Physical …, 2024 - ACS Publications
In-droplet hydrogen/deuterium exchange (HDX)-mass spectrometry (MS) experiments have
been conducted for peptides of highly varied conformational type. A new model is presented …

Development of cVSSI-APCI for the improvement of ion suppression and matrix effects in complex mixtures

ME Pursell, D Sharif, A DeBastiani, C Li… - Analytical …, 2022 - ACS Publications
The new ionization technique termed vibrating sharp-edge spray ionization (cVSSI) has
been coupled with corona discharge to investigate atmospheric pressure chemical …

Accessing Different Protein Conformer Ensembles with Tunable Capillary Vibrating Sharp-Edge Spray Ionization

D Sharif, VK Dewasurendra, MN Sultana… - The Journal of …, 2025 - ACS Publications
Capillary vibrating sharp-edge spray ionization (cVSSI) has been used to control the droplet
charging of nebulized microdroplets and monitor effects on protein ion conformation makeup …