The immunogenicity of transplanted allogeneic cells and tissues is a major hurdle to the
advancement of cell therapies. Here we show that the overexpression of eight …

The mechanisms underlying the low efficiency of reprogramming somatic cells into induced
pluripotent stem (iPS) cells are poorly understood. There is a clear need to study whether …

The plasticity of pluripotent stem cells provides new possibilities for studying development,
degeneration, and regeneration. Protocols for the differentiation of retinal organoids from …

Differentiation of epithelial cells and morphogenesis of epithelial tubes or layers is closely
linked with the establishment and remodeling of the apical junctional complex, which …

The development of technologies that allow the stable delivery of large genomic DNA
fragments in mammalian systems is important for genetic studies as well as for applications …

Repair of interstrand crosslinks (ICLs) requires the coordinated action of the intra-S-phase
checkpoint and the Fanconi anaemia pathway, which promote ICL incision, translesion …

Transgenesis is a cornerstone of molecular biology. The ability to integrate a specifically
engineered piece of DNA into the genome of a living system is fundamental to our efforts to …

Transposable elements (such as the P-element and piggyBac) have been used to introduce
thousands of transgenic constructs into the Drosophila genome. These transgenic constructs …

Fluorescent transcriptional reporters are widely used as signaling reporters and biomarkers
to monitor pathway activities and determine cell type identities. However, a large amount of …

Most animal genomes are diploid, and mammalian development depends on specific
adaptations that have evolved secondary to diploidy. Genomic imprinting and dosage …