Life exists in three dimensions, but until the turn of the century most electron microscopy
methods provided only 2D image data. Recently, electron microscopy techniques capable of …

Microscopy has gone hand in hand with the study of living systems since van Leeuwenhoek
observed living microorganisms and cells in 1674 using his light microscope. A spectrum of …

Electron microscopy (EM) achieves the highest spatial resolution in protein localization, but
specific protein EM labeling has lacked generally applicable genetically encoded tags for in …

The rules of nucleic acid base-pairing have been used to construct nanoscale architectures
and organize biomolecules, but little has been done to apply this technology in vivo. We …

Electron microscopy played a key role in establishing cell biology as a discipline, by
producing fundamental insights into cellular organization and ultrastructure. Many seminal …

Electron microscopy (EM) is the premiere technique for high-resolution imaging of cellular
ultrastructure. Unambiguous identification of specific proteins or cellular compartments in …

The review summarizes the abundant information on the 35 identified peptidoglycan (PG)
hydrolases of Escherichia coli classified into 12 distinct families, including mainly …

The electron microscope has contributed deep insights into biological structure since its
invention nearly 80 years ago. Advances in instrumentation and methodology in recent …

Electron cryotomography (ECT) enables intact cells to be visualized in 3D in an essentially
native state to'macromolecular'(∼ 4 nm) resolution, revealing the basic architectures of …

Viruses recruit cellular membranes and subvert cellular proteins involved in lipid
biosynthesis to build viral replicase complexes and replication organelles. Among the lipids …