The role of thiols in antioxidant systems
The sulfur biochemistry of the thiol group endows cysteines with a number of highly
specialized and unique features that enable them to serve a variety of different functions in …
specialized and unique features that enable them to serve a variety of different functions in …
Biological iron-sulfur clusters: Mechanistic insights from mass spectrometry
JC Crack, NE Le Brun - Coordination Chemistry Reviews, 2021 - Elsevier
Iron-sulfur (FeS) clusters are protein cofactors that are ubiquitous in life, performing a wide
range of functions, such as in electron transfer, catalysis and gene regulation. This functional …
range of functions, such as in electron transfer, catalysis and gene regulation. This functional …
The di-iron protein YtfE is a nitric oxide-generating nitrite reductase involved in the management of nitrosative stress
JC Crack, BK Balasiny, SP Bennett… - Journal of the …, 2022 - ACS Publications
Previously characterized nitrite reductases fall into three classes: siroheme-containing
enzymes (NirBD), cytochrome c hemoproteins (NrfA and NirS), and copper-containing …
enzymes (NirBD), cytochrome c hemoproteins (NrfA and NirS), and copper-containing …
Sensing mechanisms of iron–sulfur cluster regulatory proteins elucidated using native mass spectrometry
JC Crack, E Gray, NE Le Brun - Dalton Transactions, 2021 - pubs.rsc.org
The ability to sense and respond to various key environmental cues is important for the
survival and adaptability of many bacteria, including pathogens. The particular sensitivity of …
survival and adaptability of many bacteria, including pathogens. The particular sensitivity of …
Native mass spectrometry of iron-sulfur proteins
JC Crack, NE Le Brun - Fe-S proteins: methods and protocols, 2021 - Springer
Iron-sulfur clusters constitute a large and widely distributed group of protein cofactors that
play key roles in a wide range of metabolic processes. The inherent reactivity of iron-sulfur …
play key roles in a wide range of metabolic processes. The inherent reactivity of iron-sulfur …
[HTML][HTML] The Bacillus subtilis monothiol bacilliredoxin BrxC (YtxJ) and the Bdr (YpdA) disulfide reductase reduce S-bacillithiolated proteins
The bacterial cytosol is generally a reducing environment with protein cysteine residues
maintained in their thiol form. The low molecular weight thiol bacillithiol (BSH) serves as a …
maintained in their thiol form. The low molecular weight thiol bacillithiol (BSH) serves as a …
Synergy of native mass spectrometry and other biophysical techniques in studies of iron‑sulfur cluster proteins and their assembly
JC Crack, NE Le Brun - Biochimica et Biophysica Acta (BBA)-Molecular …, 2024 - Elsevier
The application of mass spectrometric methodologies has revolutionised biological
chemistry, from identification through to structural and conformational studies of proteins and …
chemistry, from identification through to structural and conformational studies of proteins and …
Metals in Motion: Understanding Labile Metal Pools in Bacteria
JD Helmann - Biochemistry, 2025 - ACS Publications
Metal ions are essential for all life. In microbial cells, potassium (K+) is the most abundant
cation and plays a key role in maintaining osmotic balance. Magnesium (Mg2+) is the …
cation and plays a key role in maintaining osmotic balance. Magnesium (Mg2+) is the …
N-methyl-bacillithiol, a Novel Thiol from Anaerobic Bacteria
ABSTRACT J. Hiras, SV Sharma, V. Raman, RAJ Tinson, et al.(mBio 9: e01603-18, 2018,
https://doi. org/10.1128/mBio. 01603-18) report on the identification of a novel thiol, N-methyl …
https://doi. org/10.1128/mBio. 01603-18) report on the identification of a novel thiol, N-methyl …
[PDF][PDF] N-methyl-bacillithiol, a novel thiol from anaerobic bacteria. mBio. 10: e02634-18
ABSTRACT J. Hiras, SV Sharma, V. Raman, RAJ Tinson, et al.(mBio 9: e01603-18, 2018,
https://doi. org/10.1128/mBio. 01603-18) report on the identification of a novel thiol, N-methyl …
https://doi. org/10.1128/mBio. 01603-18) report on the identification of a novel thiol, N-methyl …