[HTML][HTML] Detection of on-target and off-target mutations generated by CRISPR/Cas9 and other sequence-specific nucleases
The development of customizable sequence-specific nucleases such as TALENs, ZFNs and
the powerful CRISPR/Cas9 system has revolutionized the field of genome editing. The …
the powerful CRISPR/Cas9 system has revolutionized the field of genome editing. The …
Efficient targeted multiallelic mutagenesis in tetraploid potato (Solanum tuberosum) by transient CRISPR-Cas9 expression in protoplasts
M Andersson, H Turesson, A Nicolia, AS Fält… - Plant cell reports, 2017 - Springer
Key message Altered starch quality with full knockout of GBSS gene function in potato was
achieved using CRISPR-Cas9 technology, through transient transfection and regeneration …
achieved using CRISPR-Cas9 technology, through transient transfection and regeneration …
Technologies and computational analysis strategies for CRISPR applications
The CRISPR-Cas system offers a programmable platform for eukaryotic genome and
epigenome editing. The ability to perform targeted genetic and epigenetic perturbations …
epigenome editing. The ability to perform targeted genetic and epigenetic perturbations …
[HTML][HTML] Genome-scale exon perturbation screens uncover exons critical for cell fitness
CRISPR-Cas technology has transformed functional genomics, yet understanding of how
individual exons differentially shape cellular phenotypes remains limited. Here, we …
individual exons differentially shape cellular phenotypes remains limited. Here, we …
Epigenomic profiling of primary gastric adenocarcinoma reveals super-enhancer heterogeneity
Regulatory enhancer elements in solid tumours remain poorly characterized. Here we apply
micro-scale chromatin profiling to survey the distal enhancer landscape of primary gastric …
micro-scale chromatin profiling to survey the distal enhancer landscape of primary gastric …
A simple and efficient method for CRISPR/Cas9-induced mutant screening
The clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-
associated protein 9 (Cas9) system provides a technological breakthrough in mutant …
associated protein 9 (Cas9) system provides a technological breakthrough in mutant …
Genotyping genome‐edited mutations in plants using CRISPR ribonucleoprotein complexes
Z Liang, K Chen, Y Yan, Y Zhang… - Plant Biotechnology …, 2018 - Wiley Online Library
Despite the great achievements in genome editing, accurately detecting mutations induced
by sequence‐specific nucleases is still a challenge in plants, especially in polyploidy plants …
by sequence‐specific nucleases is still a challenge in plants, especially in polyploidy plants …
Adaptation to spindle assembly checkpoint inhibition through the selection of specific aneuploidies
MAY Adell, TC Klockner, R Höfler… - Genes & …, 2023 - genesdev.cshlp.org
Both the presence of an abnormal complement of chromosomes (aneuploidy) and an
increased frequency of chromosome missegregation (chromosomal instability) are …
increased frequency of chromosome missegregation (chromosomal instability) are …
A simplified method for identifying early CRISPR-induced indels in zebrafish embryos using High Resolution Melting analysis
É Samarut, A Lissouba, P Drapeau - BMC genomics, 2016 - Springer
Abstract Background The CRISPR/Cas9 system has become a regularly used tool for editing
the genome of many model organisms at specific sites. However, two limiting steps arise in …
the genome of many model organisms at specific sites. However, two limiting steps arise in …
MARCH6 and TRC8 facilitate the quality control of cytosolic and tail‐anchored proteins
S Stefanovic‐Barrett, AS Dickson, SP Burr… - EMBO …, 2018 - embopress.org
Misfolded or damaged proteins are typically targeted for destruction by proteasome‐
mediated degradation, but the mammalian ubiquitin machinery involved is incompletely …
mediated degradation, but the mammalian ubiquitin machinery involved is incompletely …