2-aminopurine as a fluorescent probe of DNA conformation and the DNA–enzyme interface
Nearly 50 years since its potential as a fluorescent base analogue was first recognized, 2-
aminopurine (2AP) continues to be the most widely used fluorescent probe of DNA structure …
aminopurine (2AP) continues to be the most widely used fluorescent probe of DNA structure …
Accurate prediction of protein function using statistics-informed graph networks
Understanding protein function is pivotal in comprehending the intricate mechanisms that
underlie many crucial biological activities, with far-reaching implications in the fields of …
underlie many crucial biological activities, with far-reaching implications in the fields of …
Sequence, structure and functional diversity of PD-(D/E) XK phosphodiesterase superfamily
K Steczkiewicz, A Muszewska, L Knizewski… - Nucleic acids …, 2012 - academic.oup.com
Proteins belonging to PD-(D/E) XK phosphodiesterases constitute a functionally diverse
superfamily with representatives involved in replication, restriction, DNA repair and tRNA …
superfamily with representatives involved in replication, restriction, DNA repair and tRNA …
[图书][B] DNA and RNA modification enzymes: structure, mechanism, function and evolution
H Grosjean - 2009 - taylorfrancis.com
This volume is a timely and comprehensive description of the many facets of DNA and RNA
modification-editing processes and to some extent repair mechanisms. Each chapter offers …
modification-editing processes and to some extent repair mechanisms. Each chapter offers …
Crystal structure and mechanism of action of the N6-methyladenine-dependent type IIM restriction endonuclease R. DpnI
W Siwek, H Czapinska, M Bochtler… - Nucleic acids …, 2012 - academic.oup.com
DNA methylation-dependent restriction enzymes have many applications in genetic
engineering and in the analysis of the epigenetic state of eukaryotic genomes. Nevertheless …
engineering and in the analysis of the epigenetic state of eukaryotic genomes. Nevertheless …
Defining the Role of Nucleotide Flipping in Enzyme Specificity Using 19F NMR
A broad range of proteins employ nucleotide flipping to recognize specific sites in nucleic
acids, including DNA glycosylases, which remove modified nucleobases to initiate base …
acids, including DNA glycosylases, which remove modified nucleobases to initiate base …
Structure of the EndoMS-DNA complex as mismatch restriction endonuclease
S Nakae, A Hijikata, T Tsuji, K Yonezawa, K Kouyama… - Structure, 2016 - cell.com
Archaeal NucS nuclease was thought to degrade the single-stranded region of branched
DNA, which contains flapped and splayed DNA. However, recent findings indicated that …
DNA, which contains flapped and splayed DNA. However, recent findings indicated that …
On the divalent metal ion dependence of DNA cleavage by restriction endonucleases of the EcoRI family
V Pingoud, W Wende, P Friedhoff, M Reuter… - Journal of molecular …, 2009 - Elsevier
Restriction endonucleases of the PD… D/EXK family need Mg2+ for DNA cleavage.
Whereas Mg2+ (or Mn2+) promotes catalysis, Ca2+ (without Mg2+) only supports DNA …
Whereas Mg2+ (or Mn2+) promotes catalysis, Ca2+ (without Mg2+) only supports DNA …
Statistical analysis of structural determinants for protein–DNA‐binding specificity
ABSTRACT DNA‐binding proteins play critical roles in biological processes including gene
expression, DNA packaging and DNA repair. They bind to DNA target sequences with …
expression, DNA packaging and DNA repair. They bind to DNA target sequences with …
Structure and cleavage activity of the tetrameric MspJI DNA modification-dependent restriction endonuclease
JR Horton, MY Mabuchi, D Cohen-Karni… - Nucleic acids …, 2012 - academic.oup.com
The MspJI modification-dependent restriction endonuclease recognizes 5-methylcytosine or
5-hydroxymethylcytosine in the context of CNN (G/A) and cleaves both strands at fixed …
5-hydroxymethylcytosine in the context of CNN (G/A) and cleaves both strands at fixed …