Cryo-EM structure of the complete inner kinetochore of the budding yeast point centromere
The point centromere of budding yeast specifies assembly of the large kinetochore complex
to mediate chromatid segregation. Kinetochores comprise the centromere-associated inner …
to mediate chromatid segregation. Kinetochores comprise the centromere-associated inner …
Stable centromere association of the yeast histone variant Cse4 requires its essential N-terminal domain
Chromosome segregation relies on kinetochores that assemble on specialized centromeric
chromatin containing a histone H3 variant. In budding yeast, a single centromeric …
chromatin containing a histone H3 variant. In budding yeast, a single centromeric …
Concatemer-assisted stoichiometry analysis: targeted mass spectrometry for protein quantification
Large multiprotein machines are central to many biological processes. However,
stoichiometric determination of protein complex subunits in their native states presents a …
stoichiometric determination of protein complex subunits in their native states presents a …